Chromatin Antibody

Label Mnemonic:	CHROM
Epic code:	LAB7827
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	86235

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3.0 mL whole blood from light green top tube or TWO Microtainer^® devices

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

3 hours (upon receipt in laboratory)

Reference Range:

1.0 AI (antibody index) or less

Interpretive Data:

Antibodies to chromatin (DNA complexed with histone proteins) are found in >50% of patients with systemic lupus erythematosus (SLE), and are significantly associated with kidney disease in this patient group. Chromatin antibodies may be found in patients with drug-induced lupus. Chromatin antibodies may also be found in patients with autoimmune hepatitis, and Sjogren's syndrome.

Comments:

New assay introduced February 25, 2013.

Methodology:

Multiplex flow immunoassay

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

CPT Code:

86235

See also:
Anti-Nuclear Antibody Screen and Reflex Titer by IFA, Serum
Double Stranded DNA Antibody, Plasma
RNP Antibody, Plasma
Ribosomal P Protein, Plasma
SS-A Antibody, Plasma
SS-B Antibody, Plasma
Sm/RNP (Common Motif) Antibodies, Plasma