Anti-Nuclear Antibody (ANA) Screen with Reflex Titer
Order Code: ANAS
Order Form: Immunopathology Requisition
Specimen:
Serum
Collection Medium:
Red top tube
Minimum:
Adult and pediatric - 2 mL; red top tube
Testing Schedule:
Daily - Batch analysis performed daily excluding weekends and university holidays.
Analytic Time:
3 days
Reference Range:
<1:40 for screen and titer
Comments:
If the screen is positive at 1:40, ANA titer will be performed. Sera positive at 1:80 or greater will be stored in serum bank for one year if additional ENA testing is necessary. ANA screen will detect centromere and most other specificities, but may not detect Anti-SS-A- (Ro) Antibody.
Methodology:
Indirect Immunofluorescence on Hep 2 cells
Sample Processing:
Centrifuge at 3500 RPM for 5 minutes.
Aliquot serum into labeled container and cap.
Sample Storage:
Refrigerate serum 2-8°C.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.
CPT Code:
86038 ANA screen 86039 ANA titer