Specimen:

Serum

Collection Medium:

Minimum:

Adult and pediatric - 2 ml; red top tube

Testing
Schedule:

Daily - Batch analysis performed daily excluding 
weekends and university holidays.

Analytic Time:

3 days

Reference Range:

<1:40 for screen and titer

Comments:

If the screen is positive at 1:40, ANA titer will be performed.  
Fluorescent pattern(s) will be reported.  Sera positive at 1:80 
dilution will be held for approximately three months in case further 
testing is required.  Sera positive at 1:320 or greater will be stored 
in serum bank for approximately six months.  ANA screen will detect 
centromere and most other specificities, but may not detect 
Anti-SS-A-(Ro) Antibody.

Methodology:

Indirect Immunofluorescence on Hep 2 cells

Sample
Processing:

Centrifuge at 3500 RPM for 5 minutes.
Aliquot serum into labeled container and cap.

Sample
Storage:

Refrigerate serum 2-8 degree C.

Transport
Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at ambient temperature.

CPT Code:

86038 ANA screen
86039 ANA titer