Cryoglobulin, Qual with Reflex to IFE Typing & Immunoglobulins
Commercial Mail-out Laboratory
Serum (in two pre-warmed tubes)
It is critical to keep specimen warm
(37°C) during transport. Keep specimen at body temperature
under arm) and deliver immediately to Specimen Control. Spin within
one hour of collection time.
Fasting specimen required.
|Red top tube 5 mL (Clot Activator)
||Red top tube 5 mL (Clot Activator)
3 mL serum in red top tube
Proper collection and transport of specimen
is critical to the outcome of the assay. Quantities less than 3 mL may
affect the sensitivity of the assay.
Plasma. Serum separator tubes. Grossly hemolyzed or lipemic
3-5 days upon receipt at reference laboratory.
Cryoglobulin, Qualitative - Negative at 72 hours.
Immunofixation Electrophoresis Gel - Normal IFE
Immunoglobulin A, Cryoprecipitate - 0 mg/dL
Immunoglobulin G, Cryoprecipitate - 0 mg/dL
Immunoglobulin M, Cryoprecipitate - 0 mg/dL
Note: Cryoglobulins are usually associated with certain plasma cell
and lymphoproliferative disorders, but have also been demonstrated in
collagen vascular disease, hepatitis C infection, and infections such
as infectious mononucleosis and cytomegalovirus disease.
If Cryoglobulin Qualitative is positive, then Immunofixation
Electrophoresis Typing and Quantitative IgA, IgG and IgM will be
added. Additional charges apply.
If no IFE is desired order test CRYO (LAB1294). Incubate unspun red
top tubes at 37°C for 45 minutes, if clotted remove. If not
clotted incubate for an additional 15 minutes at 37°C. Total
incubation time is 1 hour (do not exceed). Spin at room temperature
and aliquot 1.5 mL each into 2 aliquot tubes. Store and send room
temperature. If only 1 red top received continue with process and
note "1 tube received".
Qualitative Cold Precipitation/Qualitative Immunofixation
82595 Cryoglobulin; if reflexed for positive cryoglobulins, add 86334
for IFE typing and 82784 x3 for the quantitative IgA, IgG, and IgM
concentrations on the cryoprecipitate.