Enterovirus Qualitative PCR Assay
Label Mnemonic: ENTEROQAL
Epic Lab Code: LAB2240
Downtime Form: A-1a Clinical Microbiology Laboratory Requisition
Microbiology/Molecular Infectious Disease
BT 6004
356-2591
Specimen(s):
Spinal Fluid (CSF)
Specimen Instructions:
Sterility must be maintained for accurate results. This test is performed on CSF only.
Collection Medium:
CSF collection kit
Minimum:
0.5 mL
Delivery Instructions:
Keep on ice and deliver to laboratory immediately.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
3 hours (upon receipt in laboratory)
Reference Range:
Not detected
Comments:
The Xpert EV Assay is designed to detect enterovirus (EV) RNA (enterovirus genome 5' untranslated region between nucleotide 452 and 596) in CSF samples. The assay detects most enteroviruses, including echoviruses, coxsackie A viruses, and coxsackie B viruses. The assay does not distinguish between serotypes.

Results from the Xpert EV assay should be interpreted in conjunction with other laboratory and clinical data. Positive enterovirus PCR results do not rule out other causes of meningitis, including bacteria, mycobacteria, other viruses (herpes family viruses, arboviruses, mumps virus, etc) and fungi. Rare occurrences of simultaneous mixed bacterial- viral meningitis have been reported in the literature.

This test is only performed on CSF. For detection of enteroviruses in blood, order Enterovirus Detection by RTPCR.
Methodology:
Real-time Reverse transcriptase PCR amplification; Xpert EV Test (Cepheid)
CPT Code:
87498