Enterovirus Detection by RTPCR , Plasma

Enterovirus Detection by RTPCR

Order Code:	EVPCR
Epic Lab Code:	LAB4802
Order Form:	A-1a Miscellaneous Request or Epic Req

Commercial Mail-out Laboratory
6240 RCP
356-3527

Specimen:

Plasma

Collection Medium:

Lavender top tube 4 mL (EDTA)

Alternate Collection Media:

Pink top tube

Minimum:

Preferred minimum: 4 mL lavender (EDTA) or pink (K2EDTA) Absolute minimum: 1 mL plasma in a sterile container Pediatric minimum: 0.5 mL plasma in a sterile container (Less volume may decrease sensitivity of assay.)

Rejection Criteria:

Nonfrozen samples, samples exposed to repeated freeze/thaw cycles, nonsterile or leaking containers, heparinized samples, and hemolyzed samples.

Delivery Instructions:

Deliver to laboratory within 1 hour of collection.

Analytic Time:

1 week upon receipt at reference laboratory

Reference Range:

Negative - Enterovirus nucleic acid not detected by RT-PCR Positive - Enterovirus nucleic acid detected by RT-PCR

Interpretive Data:

A negative result does not rule out the presence of PCR inhibitors in the patient specimen or Enterovirus nucleic acid in concentrations below the level of detection of the assay.

Methodology:

Reverse Transcription/Polymerase Chain Reaction

CPT Code:

87498