Specimen

Peripheral Blood

Collection Medium:

Alternate
Collection Media:

Green top tube (Na Heparin), Lavender top tube (EDTA)

Minimum:

Adult:  10 mL whole blood in a yellow top tube (ACD solution A)
Pediatric:  2 mL whole blood in a yellow top tube (ACD solution A)
Minimum specimen requirements are cell count dependent.

For absolute quantitative results, a CBC with Automated Differential 
must also be ordered.

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.

Testing Schedule:

0800-1630 Monday through Friday.  For additional services, 
contact Clinical Pathology Resident on-call at pager #3404.

Analytic Time:

2 days

Reference Range:

Antibodies performed vary with the patient's clinical problem; please 
provide history and lab findings. The pathologist will provide an 
interpretative report.

Adult Immunodeficiency Screening Panel:
CD3, CD4, CD8, CD14, CD19, CD25, CD45, and CD56

Autoimmune Lymphoproliferative Syndrome (ALPS):
CD3, CD4, CD5, CD8, CD14, CD16+56, CD19, CD20, CD45, CD56, CD95, 
HLA-DR, TCR alpha-beta, TCR gamma-delta, Kappa, and Lambda

Bruton's Agammaglobulinemia:
CD3/CD4/CD8, CD5/CD20, CD14/CD45, CD19/Kappa, CD19/Lambda

Comments:

Specimens with absolute lymphocyte counts of <100/mm3 can not be
tested.

Test includes various T and B cell assays, as dictated by the clinical 
question asked.  Include pertinent clinical information on the 
reqisition.  Deliver specimen immediately to Immunopathology Lab to
insure leukocyte viability.  Do not deliver to specimen control area.
Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

88184, 88185 variable - Technical
88187, 88188 or 88189 variable - Professional (varies due to the number 
of antibodies performed)

See Additional Information:
Specimens Requiring Immediate Delivery