Immunodeficiency Evaluations; Adult and Pediatric
Order Form: A-1a Immunopathology or Epic Req
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009
Tube Station #260
Specimen:
Peripheral Blood
Collection Medium:
Yellow top tube (ACD solution A)
Alternate Collection Media:
Green top tube 4 mL (Na Heparin), Lavender top tube 3 mL (EDTA)
Minimum:
Adult: 10 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a yellow top tube (ACD solution A)
Minimum specimen requirements are cell count dependent.

For absolute quantitative results, a CBC with Automated Differential must also be ordered.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.
Testing Schedule:
0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.
Analytic Time:
2 days
Reference Range:
Antibodies performed vary with the patient's clinical problem; please provide history and lab findings. The pathologist will provide an interpretative report. Adult Immunodeficiency Screening Panel: CD3, CD4, CD8, CD14, CD19, CD25, CD45, and CD56 Autoimmune Lymphoproliferative Syndrome (ALPS): CD3, CD4, CD5, CD8, CD14, CD16+56, CD19, CD20, CD45, CD56, CD95, HLA-DR, TCR alpha-beta, TCR gamma-delta, Kappa, and Lambda Bruton's Agammaglobulinemia: CD3/CD4/CD8, CD5/CD20, CD14/CD45, CD19/Kappa, CD19/Lambda
Comments:
Specimens with absolute lymphocyte counts of <100/mm3 can not be tested. Test includes various T and B cell assays, as dictated by the clinical question asked. Include pertinent clinical information on the reqisition. Deliver specimen immediately to Specimen Control. Recent corticosteroid or chemotherapy may invalidate result.
Methodology:
Flow Cytometry-Whole Blood Lysis
CPT Code:
88184, 88185 variable - Technical 88187, 88188 or 88189 variable - Professional (varies due to the number of antibodies performed)
 
See Additional Information:
Specimens Requiring Immediate Delivery