Specimen

Whole Blood

Collection Medium:

Minimum:

Full draw; any size lavender top or fingerstick.

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Analytic Time:

Routine turn around time is approximately 2.5 hours.

Reference Range:

                         Adults         Children (5-18 yrs)
                          (mm3)                (mm3)
Band Neutrophils          -                   -
Neutrophils           2188-7800/mm3       1700-7500/mm3
Eosinophils             40-390/mm3          40-650/mm3
Basophils               10-136/mm3           7-140/mm3
Monocytes              130-860/mm3          28-825/mm3
Lymphocytes            875-3300/mm3       1250-3380/mm3
Immature Granulocytes     -                   -

Comments:

A CBC must be ordered to perform a differential.  Hematology 
instruments are automated differential screening instruments that 
identify WBC's by their size and staining activity.  It performs a 
complete cell identification that includes neutrophils, lymphocytes, 
monocytes, basophils and eosinophils. The automated instruments count 
32,000 white blood cells and statistically provide a more accurate 
differential count than a manual differential.  All differentials will 
be analyzed on the instrument first.  If the results fail the screening 
criteria, a manual differential will be performed.  Pathologist 
approval is required to perform a manual differential on a specimen 
with an automated differential that passed the screening criteria.

Please refer to the PowerPoint Presentation for further information.

Methodology:

Flow Cytometry
Auto Diff Cytochemical Staining
Manual Diff Wright's Stain

CPT Code:

85025

See Additional Information:
Hematology Pediatric Reference Ranges