Lung NSCLC Targeted RNA Fusions with Interpretation
Label Mnemonic: CTL LUNG
Epic code: LAB8567
Order form: Molecular Oncology Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 81445
Formalin-fixed, paraffin embedded tissue block, or ten unstained 4 micron-thick sections on non-plus slides and one H&E stained section
Rejection Criteria:
Specimens that have been decalcified will not be accepted.
Turn Around Time:
10-14 days
Reference Range:
Fusion or mutation not detected
Tissue sections are reviewed by a pathologist. RNA is isolated from the selected area of the sample. Anchored multiplex PCR for targeted next-generation sequencing is performed. The sequenced sample is a reverse transcription PCR-amplified fragment library in which each sample is uniquely identified by ligation of a short oligonucleotide barcode. The resultant sequence identifies the exons of the fusion transcript arising from the specific gene target and the partner gene.

The panel targets gene rearrangements of these gene targets: AKT1, ALK, AXL, BRAF, CCND1, EGFR (vIII), FGFR1, FGFR2, FGFR3, MET (exon 14 skip), NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, ROS, and THADA.

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Massively parallel next generation sequencing on RNA
Sample Storage:
Room Temperature.
Transport Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Recommend early AM overnight shipping or equivalent if not on courier service.
Ship at room temperature.
Protect block or slides from excessive heat and ship in cooled container during summer months.
CPT Code: