Pan-Solid Tumor RNA Fusion Panel

Label Mnemonic:	PANST
Epic code:	LAB9492
Order form:	Molecular Oncology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	81456

Specimen(s):

Formalin-fixed, paraffin embedded tissue block, or ten unstained 4 micron-thick sections on non-plus slides and one H&E stained section

Minimum:

One tumor-containing formalin-fixed paraffin embedded tissue block or 10 unstained 4 micron-thick sections on non-plus slides and one H&E stained section from one tumor-containing FFPE block or any or all unstained or stained slides from tumor-containing fine needle aspirate cytologic preparations.

Rejection Criteria:

Specimens that have been decalcified will not be accepted.

Turn Around Time:

10-14 days

Reference Range:

Fusion or mutation not detected

Comments:

Tissue sections are reviewed by a pathologist. RNA is isolated from the selected area of the sample. Anchored multiplex PCR for targeted next-generation sequencing is performed. The sequenced sample is a reverse transcription PCR-amplified fragment library in which each sample is uniquely identified by ligation of a short oligonucleotide barcode. The resultant sequence identifies the exons of the fusion transcript arising from the specific gene target and the partner gene.

The panel targets gene rearrangements of these gene targets: ACVR2A, AKT1, AKT2, AKT3, ALK, AR, ARHGAP26, ARHGAP6, ATF1, AXL, BCOR, BEND2, BRAF, BRD3, BRD4, C11orf95, CALCA, CAMTA1, CCNB3, CCND1, CD274, CIC, CREB1, CREM, CRTC1, CSF1, CSF1R, CTNNB1, DDR2, DNAJB1, EGF, EGFR, EPC1, ERBB2, ERBB4, ERG, ESR1, ESRRA, ETV1, ETV4, ETV5, ETV6, EWSR1, FGF1, FGFR1, FGFR2, FGFR3, FGR, FOS, FOSB, FOXO1, FOXO4, FOXR2, FUS, GLI1, GNAS, GRB7, HMGA2, HRAS, IDH1, IDH2, IGF1R, INSR, JAK2, JAK3, JAZF1, KIT, KRAS, KRT20, KRT7, MAML2, MAP2K1, MAST1, MAST2, MBTD1, MDM2, MEAF6, MERTK, MET, MGEA5, MKL2, MN1, MSMB, MUSK, MYB, MYBL1, MYC, MYOD1, NCOA1, NCOA2, NCOA3, NFATC2, NFE2L2, NFIB, NOTCH1, NOTCH2, NR4A3, NR4A3, NRAS, NRG1, NTRK1, NTRK2, NTRK3, NUMBL, NUTM1, PAX3, PAX8, PDGFB, PDGFD, PDGFRA, PDGFRB, PHF1, PHKB, PIK3CA, PKN1, PLAG1, PPARG, PRDM10, PRKACA, PRKACB, PRKCA, PRKCB, PRKCD, PRKD1, PRKD2, PRKD3, PTH, RAD51B, RAF1, RELA, RET, ROS1, RSPO2, RSPO3, SLC5A5, SS18, SS18L1, STAT6, TAF15, TCF12, TEK, TERT, TFE3, TFEB, TFG, TGFA, THADA, TMPRSS2, TTF1, USP6, VGLL2, WWTR1, YAP1, YWHAE

Methodology:

Massively parallel next generation sequencing on RNA

Sample Processing:

Formalin-fixed, paraffin embedded tissue block, or ten unstained 4 micron-thick sections on non-plus slides and one H&E stained section.

Sample Storage:

Room Temperature.

Transport Instructions:

Formalin-fixed paraffin embedded blocks or slides ship at room temperature.
Place completed laboratory requisition (or packing list) into outside pocket of bag.
Protect block or slides from excessive heat and ship in cooled container during summer months.
Ship at room temperature.