Sarcoma Fusion Panel with Interpretation
Epic code: LAB8427
Order form: Molecular Oncology Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 81445
Specimen(s):
Formalin Fixed, Paraffin Embedded Tissue Block
10 unstained 5-10 μM slides at 20-25°C and an H&E slide of the tissue
Minimum:
Tumor cells more than 50% of the total tissue and greater than 10mm2 in surface area on the block.
Rejection Criteria:
Specimens fixed in B5 fixative or that have been decalcified will not be accepted. Tumor specimens containing less than 50% tumor cells or are less than 10mm2 in area may be unacceptable.
Testing Schedule:
Weekly
Turn Around Time:
21 days
Comments:
The Sarcoma Fusion Panel uses massively parallel next generation RNA sequencing that can detect and identify fusions of 24 genes associated with soft tissue cancers.

Gene Fusions Assessed (Those in bold may be ordered individually):
Disease                                         Gene
Aneurysmal bone cyst                            USP6
Adamantinoma                                    NCOA2
Alveolar rhabdomyosarcoma (FKHR)                FOXO1
Alveolar soft part, RCC                         TFE3
Chondroid lipoma                                MKL2
Chordoma                                        HMGA2
Congenital fibrosarcoma                         NTRK3
Dermatofibrosarcoma protuberans                 PDGFB
Endometrial stroma tumor                        JAZF1
Endothelial stromal sarcoma (PHF1)              EPC1
Epithelioid hemangioendothelioma                CAMTA1
Endometrial Stromal Sarcoma                     MEAF6
ESS, clear cell sarcoma of the kidney (FAM22)   YWHAE
Ewing/PNET, clear cell, DRCT,                   EWSR1
  extraskeletal myxoid chondrosarcoma
Ewing-like sarcoma (BCOR-CCNB3)                 CCNB3
Extrapleural solitary fibrous tumor             STAT6
Extraskeletal Myxoid Chondrosarcoma             TCF12
Extraskeletal myxoid chondrosarcoma             TFG
Extraskeletal Myxoid Chondrosarcoma, leukemia   TAF15
Inflammatory myofibroblastic tumor              ALK
LG fibromyxoid, myxoid liposarcoma              FUS
Lipoblastoma                                    PLAG1
Pericytoma, LG (ACTB)                           GLI1
Pigmented villonodular synovitis (COL6A3)       CSF1
Synovial Sarcoma                                SS18
Undifferentiated small cell sarcoma (DUX4)      CIC
Methodology:
Massively parallel Next Generation RNA sequencing by synthesis, semiconductor technology, Ion Torrent, Personal Genomics Machine.
Instructions:
Submit formalin fixed, paraffin embedded tissue block and an H&E slide with the requisition. Please include the surgical pathology report and relevant clinical information in order to provide correct interpretation of test results.
Transport Instructions:
Formalin-fixed paraffin embedded blocks or slides ship at room temperature.
Place requisition into outside pocket of bag.
Protect block from excessive heat and ship in cooled container during summer months.
CPT Code:
81445