Herpes Simplex Virus by PCR
Label Mnemonic: HSVPCRB
Epic code: LAB7879
Order form: Laboratory Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 87529
Specimen(s):
Whole Blood
Collection Medium:
Lavender top tube 3 mL (EDTA)
Minimum:
2 mL whole blood collected in a lavender (EDTA) top tube.

For pediatric collection, collect FOUR full lavender top Microtainer® (EDTA) tubes (Absolute Minimum: THREE full Microtainer® tubes).
Rejection Criteria:
Heparinized specimens
Turn Around Time:
1-3 days upon receipt at reference laboratory
Comments:
For diagnosis of neonatal herpes simplex infection. See also HSV Qualitative PCR, for testing of neonatal surface swabs (conjunctivae, mouth, nasopharynx and rectum, and scalp electrode site if present); this test is performed in house Monday through Friday.

For disseminated disease only. Adults can reactivate HSV asymptomatically so test has limited utility without evidence of dissemination (hepatitis, chorioretinitis, etc.). PCR testing of lesions or CSF is preferred in most adult cases.
Methodology:
Qualitative Polymerase Chain Reaction
Instructions:
If specimen cannot be delivered immediately, separate plasma from cells. Transfer 1 mL plasma to sterile container and ship to UIDL frozen.
CPT Code:
87529
 
See also:
HSV Qualitative PCR, Vesicle Fluid, Surface Swab