HSV Qualitative PCR
Label Mnemonic: HSVPCR
Epic code: LAB2467
Order form: Microbiology/Molecular Infectious Disease Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 87529
Specimen(s):
Vesicle Fluid, Surface Swab (in UTM; Throat, Buccal Mucosa, Cervical or Genital Lesions), Eye swab samples collected in UTM or CSF
Collection Medium:
or or
Chlamydia/Viral Transport Kit Swab Kit Straight HSV--VZV/Viral Culture 3.0 mL CSF collection kit
Minimum:
Collect one of the following specimens:
Vesicle fluid/swab (first three days of rash) in viral transport media
-OR-
0.5 mL CSF in CSF tube from the collection kit
Rejection Criteria:
Sputum, tracheal aspirate or skin scrapings.
Vitreous fluid samples - Order mailout test Herpes Simplex Virus PCR, Vitreous (LAB7468).
Testing Schedule:
0800-1630 Monday through Friday.
Turn Around Time:
24 hours (upon receipt in laboratory)
Reference Range:
Negative

Positive results will be reported as positive for HSV Type 1, HSV Type 2, or both.
Comments:
The performance characteristics of this test were determined by the University of Iowa Microbiology and Molecular Pathology Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test is for clinical purposes. It should not be regarded as investigational or for research. The laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.
Methodology:
PCR amplification
Instructions:
Immediately place vesicle fluid, swab in viral transport media. Source of specimen is required.
Sample Processing:
Immediately place vesicle fluid or swab specimen into vial of transport media and seal tightly.
Label transport tube with two patient identifiers, date and time of collection.
Indicate specimen/fluid type on requisition.
Sample Storage:
Refrigerate.
Specimen cannot be frozen.
Transport Instructions:
Place requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
87529
 
See also:
Herpes Simplex Virus PCR, Amniotic Fluid