Jo-1 Antibody

Label Mnemonic:	JO1
Epic code:	LAB3455
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	86235

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3.0 mL whole blood from light green top tube or TWO Microtainer^® devices

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

3 hours (upon receipt in laboratory)

Reference Range:

1.0 AI (antibody index) or less

Interpretive Data:

Jo-1 antibodies are a marker for polymyositis and occur most commonly in myositis patients with interstitial lung disease. Jo-1 antibodies occur in 50% or more of patient with interstitial pulmonary fibrosis and symmetrical polyarthritis. A negative result for Jo-1 antibodies does not rule out polymyositis.

Comments:

Assay methodology and reference ranges changed February 25, 2013.

Methodology:

Multiplex flow immunoassay

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

See also:
Anti-Nuclear Antibody Screen and Reflex Titer by IFA, Serum