Anti-Nuclear Antibody Screen and Reflex Titer by IFA
| Label Mnemonic: | ANAS |
| Epic code: | LAB1256 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 86038 ANA screen 86039 ANA titer |
Specimen(s):
Serum
Collection Medium:
 |
| Red top tube 5 mL (Clot Activator) |
Minimum:
Adult and pediatric - 2 mL; red top tube
Testing
Schedule:
Daily - Batch analysis performed daily excluding
weekends and university holidays.
Turn Around
Time:
3 days
Reference Range:
<1:80 for screen and titer
Methodology:
Indirect Immunofluorescence on Hep 2 cells
Sample
Processing:
Centrifuge at 3500 RPM for 5 minutes.
Transfer serum into new labeled container and cap.
Transfer serum into new labeled container and cap.
Sample
Storage:
Refrigerate serum 2-8°C.
Transport
Instructions:
Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Place requisition into outside pocket of bag.
If the screen is positive at 1:80, ANA titer will be performed. ANA screen will detect centromere and most other specificities, but may not detect Anti-SS-A-(Ro) Antibody.