Double Stranded DNA Antibody
|Plasma Separator Tube 4.5 mL
Call laboratory for additional acceptable specimen collection containers.
3.0 mL whole blood from light green top tube or TWO
24 hrs/day, 7 days a week, including holidays.
3 hours (upon receipt in laboratory)
Negative: 4 IU/mL or less
Indeterminate: 5-9 IU/mL
Positive: 10 IU/mL or greater
Double-stranded DNA (dsDNA) IgG antibodies are part of the American
College of Rheumatology criteria for the diagnosis of systemic lupus
erythematosus (SLE). dsDNA are detectable in approximately 85% of
patients with untreated SLE and are rarely detected in other
connective tissue diseases. Weakly positive results are not specific
to SLE and can occur in other diseases. Levels of dsDNA antibodies
often fluctuate with disease activity in SLE and may decrease with
response to therapy. Increases of 25% or more in dsDNA antibody level
may indicate disease exacerbation.
Assay methodology and reference ranges changed February 25, 2013.
All first-time positive results are confirmed by Crithidia
immunofluorescence assay (IFA).
Multiplex flow immunoassay