Acute Leukemia

Order form:

Epic Beaker AP Order Label

Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260

Specimen(s):

Peripheral Blood, Bone Marrow, or CSF

Collection Medium:

Yellow top tube 8.5 mL (ACD solution A)

Alternate Collection Media:

Green top tube 4 mL (Na Heparin) or Lavender top tube 3 mL (EDTA)

Minimum:

Adult - Peripheral Blood: 10 mL; Bone Marrow:  2-4 mL

Pediatric - Peripheral Blood:  2-5 mL; Bone Marrow:  2-5 mL

CSF - Volume required is cell count dependent.  Body fluid specimens 
containing small number of leukocytes may not be adequate for 
immunophenotyping. Provide as much CSF specimen as possible in the 
original CSF collection tube(s).

Delivery Instructions:

Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.

Testing Schedule:

0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.

Turn Around Time:

2 days

Reference Range:

Antibodies routinely included are: CD1A, CD2, CD3, CD4, CD5, CD7, 
CD8, CD10, CD11B, CD13, CD14, CD15, CD16, CD19, CD20, CD22, CD33, 
CD34, CD36, CD38, CD45, CD56, CD61, CD64, CD71, CD117, HLA-DR, Kappa, 
Lambda.

Additional antibodies are added as necessary for diagnosis after 
morphologic examination. The pathologist will provide an 
interpretative report.

Comments:

Epic code:  LAB8255 for Blood, LAB8284 for Bone Marrow, LAB8254 for Other

Specimens accepted from Monday 0800 until Friday 1630.

Include pertinent clinical information on the requisition.

Additional antibodies may be necessary for diagnosis.

Test Limitations:

Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Lysed Whole Blood/Marrow

CPT Code:

Technical: 88184 (first antibody), 88185 (each additional antibody); Professional: 88189

See Additional Information:
Specimens Requiring Immediate Delivery