Cholesterol, High-Density Lipoprotein

Label Mnemonic:	HDLP
Epic code:	LAB101
Downtime form:	A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Chemistry
6240 RCP
356-3527

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or 1 Microtainer^® for pediatric patients

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

>39 mg/dL

Comments:

Fasting for at least 8 hours prior to collection is recommended.

This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).

Test Limitations:

Criterion: Recovery within plus or minus 10% of initial value.

Icterus: No significant interference up to an I index of 60.

Hemolysis: No significant interference up to an H index of 1200 
(approximate hemoglobin concentration: 1200 mg/dL).

Lipemia (Intralipid): No significant interference up to an L index of 
2000. There is poor correlation between turbidity and triglycerides 
concentration.

Ascorbic acid: No significant interference up to 50 mg/dL.

In rare cases, elevated immunoglobulin concentrations can cause 
unreliable results.  Abnormal liver function does affect lipid 
metabolism; consequently, HDL and LDL results are of limited 
diagnostic value. In some patients with abnormal liver function, the 
HDL result may significantly differ from the DCM (designated 
comparison method) result.

No interference was found at therapeutic concentrations using common 
drug panels. Statins and fibrates tested at therapeutic concentration 
ranges did not interfere.  N-acetylcysteine at the therapeutic 
concentration when used as an antidote and the acetaminophen 
metabolite N-acetyl-p-benzoquinone imine (NAPQI) independently may 
cause falsely low results.

Venipuncture immediately after or during the administration of 
metamizole may lead to falsely low results.

Methodology:

Enzymatic

Assay updated to Roche HDL Generation 4 on 06/04/18

CPT Code:

83718

See Additional Information:
Fasting Specimen Requirements