Cholesterol, High-Density Lipoprotein
Label Mnemonic: HDLP
Epic Lab Code: LAB101
Downtime Form: A-1a General Laboratory Requisition
Chemistry
6240 RCP
356-3527
Specimen(s):
Plasma
Collection Medium:
Plasma Separator Tube 4.5 mL
Alternate Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or 1 Microtainer® for pediatric patients
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
1 hour (upon receipt in laboratory)
Reference Range:
>41 mg/dL
Comments:
Fasting for at least 8 hours prior to collection is recommended.

This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).
Test Limitations:
Criterion: Recovery within plus or minus 10% of initial value. Icterus: No significant interference up to an I index of 60. Hemolysis: No significant interference up to an H index of 1200 (approximate hemoglobin concentration: 1200 mg/dL). Lipemia (Intralipid): No significant interference up to an L index of 1800. There is poor correlation between turbidity and triglycerides concentration. The claim for lipemia interference is based on the Glick model (1), which uses Intralipid as an artificial substrate. To date, there is no model available which can mimic interference by triglycerides, as triglyceride levels in patient specimens behave unpredictably, depending on the nature of the esterified fatty acids in the samples. Patient specimens with elevated triglyceride levels are very often lipemic. Therefore customers cannot verify interference by triglycerides in patient specimens. Ascorbic acid less than 50 mg/dL. In rare cases, elevated immunoglobulin concentrations can lead to falsely increased HDL-cholesterol results. Abnormal liver function does affect lipid metabolism; consequently, HDL and LDL results are of limited diagnostic value. In some patients with abnormal liver function, the HDL-C plus result is significantly negatively biased versus the DCM (designated comparison method) result. Of 17 commonly used drugs tested in vitro, none interfered with the assay. Reference: Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470-474.
Methodology:
Enzymatic
CPT Code:
83718
 
See Additional Information:
Fasting Specimen Requirements