3 mL whole blood from light green top tube or 1 Microtainer^® for pediatric patients

Guidelines from the National Lipid Association and the National Cholesterol Education Program for cardiovascular risk for adults 18 years of age and older:

Males: 40 mg/dL or higher

Females: 50 mg/dL or higher

Fasting for at least 8 hours prior to collection is recommended.

This test is also performed in the Iowa River Landing (IRL) clinical laboratory (for specimens drawn at that site).

Criterion: Recovery within plus or minus 10% of initial value.



Icterus: No significant interference up to an I index of 60.



Hemolysis: No significant interference up to an H index of 1200 

(approximate hemoglobin concentration: 1200 mg/dL).



Lipemia (Intralipid): No significant interference up to an L index of 

2000. There is poor correlation between turbidity and triglycerides 

concentration.



Ascorbic acid: No significant interference up to 50 mg/dL.



In rare cases, elevated immunoglobulin concentrations can cause 

unreliable results.  Abnormal liver function does affect lipid 

metabolism; consequently, HDL and LDL results are of limited 

diagnostic value. In some patients with abnormal liver function, the 

HDL result may significantly differ from the DCM (designated 

comparison method) result.



No interference was found at therapeutic concentrations using common 

drug panels. Statins and fibrates tested at therapeutic concentration 

ranges did not interfere.  N-acetylcysteine at the therapeutic 

concentration when used as an antidote and the acetaminophen 

metabolite N-acetyl-p-benzoquinone imine (NAPQI) independently may 

cause falsely low results.



Venipuncture immediately after or during the administration of 

metamizole may lead to falsely low results.

Enzymatic

Assay updated to Roche HDL Generation 4 on 06/04/18