Adult Minimum: 1 mL serum required 

Absolute Minimum: 0.5 mL 

Pediatric Minimum: 0.5 mL

Grossly lipemia specimens.

2-6 days upon receipt at reference laboratory

Phenotype - By report Alpha-1-Antitrypsin - 100-190 mg/dL

The interpretive report will identify the alleles present. For rare alleles, the report will indicate whether or not they have been associated with reduced quantitative levels of alpha-1-antitrypsin.

Alpha-1-Antitripysin total will also be performed. If phenotyping is not requested, then order Alpha-1-Antitrypsin Quantitation.

This assay identifies the phenotype of the circulating alpha-1-antitrypsin (A1A) protein. If the patient is already on replacement therapy, the phenotype will detect patient and replacement protein.

If 2 bands are seen, such as an M band and a Z bands, it is reported as MZ (eg, heterozygous)

If 1 band is seen, such as the Z band and the quantitative level is consistent with a homozygote, the phenotype is assumed to be homozygous and is reported as ZZ.

Interpret with caution if the patient has been transfused within the previous 21 days.

Phenotyping is done by isoelectric focusing in agarose gels.

Nephelometry.