Alpha-1-Antitrypsin Panel
Label Mnemonic: A1AP
Epic code: LAB2564
Downtime form: Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Commercial Mailout Laboratory
6240-8 RCP
356-8593
Specimen(s):
Serum
Collection Medium:
Red top tube 5 mL (Clot Activator)
Minimum:

Adult Minimum: 1 mL serum required 

Absolute Minimum: 0.5 mL 

Pediatric Minimum: 0.5 mL

Rejection Criteria:

Grossly lipemia specimens.

Turn Around Time:

2-6 days upon receipt at reference laboratory

Reference Range:

Phenotype - By report Alpha-1-Antitrypsin - 100-190 mg/dL

 

The interpretive report will identify the alleles present. For rare alleles, the report will indicate whether or not they have been associated with reduced quantitative levels of alpha-1-antitrypsin.

Comments:

Alpha-1-Antitripysin total will also be performed. If phenotyping is not requested, then order Alpha-1-Antitrypsin Quantitation.

 

This assay identifies the phenotype of the circulating alpha-1-antitrypsin (A1A) protein. If the patient is already on replacement therapy, the phenotype will detect patient and replacement protein.

 

If 2 bands are seen, such as an M band and a Z bands, it is reported as MZ (eg, heterozygous)

 

If 1 band is seen, such as the Z band and the quantitative level is consistent with a homozygote, the phenotype is assumed to be homozygous and is reported as ZZ.

Test Limitations:

Interpret with caution if the patient has been transfused within the previous 21 days.

Methodology:

Phenotyping is done by isoelectric focusing in agarose gels.

 

Nephelometry.

CPT Code:
82103 Alpha-1-Antitrypsin, Total; 82104 Alpha-1-Antitrypsin, Phenotype
 
See also:
Alpha-1-Antitrypsin Quantitation, Plasma