CD4 Lymphocytes

Label Mnemonic:	CD4/3
Epic code:	LAB1280
Downtime form:	A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260

Specimen(s):

Peripheral blood

Specimen Instructions:

Specimen is only viable for 48 hours at room temperature.

Collection Medium:

Lavender top tube 3 mL (EDTA)

Alternate Collection Media:

Pink top tube 6 mL (K2-EDTA)

Minimum:

Adult: 3 mL whole blood (Lavender) or 6 mL whole blood (Pink)

Pediatric: 2 mL whole blood

Delivery Instructions:

Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.

Testing Schedule:

0800-1630 Monday through Friday, excluding UIHC designated holidays. Must be received by 1615 before weekends or holidays. For additional services, contact Clinical Pathology Resident on-call at pager #3404.

Turn Around Time:

2 days

Reference Range:

Adult reference ranges for whole blood lysis method by flow 
cytometry:

T Cells (CD4)     34-62%     Absolute Counts:  263-2045/mm3

CD3 test is run as an internal quality assurance measure as directed 
by CDC guidelines. The results of this QA will not be charged.

Pediatric reference ranges will be provided.

Comments:

Include pertinent clinical information on the requisition. Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

86361

See Additional Information:
Specimens Requiring Immediate Delivery