Comprehensive Lung Panel
| Label Mnemonic: | LUNG DX PAN |
| Epic code: | LAB8580 |
| Downtime form: | A-1a Doctor/Provider Orders - Pathology Molecular |
Molecular Pathology
6240 RCP
384-9568
6240 RCP
384-9568
Specimen(s):
Formalin Fixed, Paraffin Embedded Tissue Block or 10 unstained 4-8 micron thick sections on non-plus slides and an H&E slide of the tissue
Minimum:
Specimen requires 20% or greater tumor cell content.
Testing Schedule:
Weekly
Turn Around
Time:
2 weeks
Comments:
This order encompasses multiple components of immunohistochemistry (PD-L1) and molecular testing for EGFR and other related DNA mutation (Iowa Cancer Mutation Profile, LAB8948), and molecular testing for RNA fusions including ALK, ROS1 and MET exon 14 skipping (Lung NSCLC RNA Fusion Panel, LAB8567).
Testing is performed from paraffin-embedded tissue of the tumor.
Substitutions and small insertion/deletions are detected in these genes: ABL1, ACVR1, AKT1, AKT2, AKT3, ALK, ANKRD26, APC, AR, ARAF, ARID1A, ATM, ATR, ATRX, AURKA, AXL, BAP1, BARD1, BIRC3, BLM, BRAF, BRCA1, BRCA2, BRIP1, BTK, CBL, CCND1, CCND2, CCND3, CCNE1, CD28, CDH1, CDK12, CDK2, CDK4, CDK6, CDK8, CDKN1A, CDKN1B, CDKN2A, CDKN2B, CHEK1, CHEK2, CIC, CREBBP, CRKL, CSF1R, CSF3R, CTNNB1, DAXX, DDR2, DDX3X, DHX15, DICER, DNMT3A, DOT1L, EED, EGFR, EPHA3, ERBB2, ERBB3, ERBB4, ERCC2, ERRFI1, ESR1, ETNK1, ETV6, EZH2, FAM175A, FANCA, FANCC, FANCD2, FANCI, FANCL, FBXW7, FGF19, FGF3, FGFR1, FGFR2, FGFR3, FGFR4, FLT1, FLT3, FOXL2, FUBP1, GATA1, GATA2, GATA3, GNA11, GNAQ, GNAS, H3F3A, HIST1H3B, HIST1H3C, HNF1A, HRAS, IDH1, IDH2, IGF1R, IGF2, JAK1, JAK2, JAK3, KDR, KIT, KLR4, KMT2D, KNSTRN, KRAS, MAGOH, MAP2K1, MAP2K2, MAP2K4, MAPK1, MAPK3, MAX, MCL1, MDM2, MDM4, MED12, MET, MLH1, MPL, MRE11A, MSH2, MSH6, MTOR, MYC, MYCL, MYCN, MYD88, NBN, NF1, NF2, NFE2L2, NFKBIA, NOTCH1, NOTCH2, NOTCH3, NRAS, NTRK1, NTRK2, NTRK3, PALB2, PARP1, PDGFRA, PDGFRB, PHF6, PIK3CA, PIK3CB, PIK3R1, PIK3R2, PLCG2, PMS2, POLD1, POLE, PPARG, PPP2R1A, PTCH1, PTEN, PTPN11, RAC1, RAD21, RAD50, RAD51, RAD51B, RAD51C, RAD51D, RAF1, RB1, RET, RHEB, RHOA, RICTOR, RNF43, ROS1, SDHA, SDHB, SDHC, SDHD, SETBP1, SETD2, SF3B1, SF3B1, SIRT2, SLIT2, SLX4, SMAD4, SMARCA4, SMARCB1, SMC1A, SMC3, SMO, SOX2, SPOP, SRC, SRSF2, STAT3, STAT5B, STK11, SUFU, TERT, TET2, TOP1, TP53, TRAF7, TSC1, TSC2, TYK2, U2AF1, U2AF2, WT1, XPO1, and ZBTB7A.
Copy number alterations (gains or losses) are detected in these genes: AKT1, AKT2, AKT3, ALK, AR, AXL, BRAF, CCND1, CCND2, CCND3, CCNE1, CDK2, CDK4, CDK6, CDKN2A, CDKN2B, EGFR, ERBB2, ESR1, FGF19, FGF3, FGFR1, FGFR2, FGFR3, FGFR4, FLT3, IGF1R, KIT, KRAS, MDM2, MDM4, MET, MYC, MYCL, MYCN, NTRK1, NTRK2, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CB, PPARG, PTEN, RICTOR, SOX2, TERT, TSC1, and TSC2.
Fusion transcripts are detected and reported involving rearrangements of these genes: AKT1, ALK, AXL, CCND1, EGFR, FGFR1, FGFR2, FGFR3, MET, NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, and ROS1.
Testing is performed from paraffin-embedded tissue of the tumor.
Substitutions and small insertion/deletions are detected in these genes: ABL1, ACVR1, AKT1, AKT2, AKT3, ALK, ANKRD26, APC, AR, ARAF, ARID1A, ATM, ATR, ATRX, AURKA, AXL, BAP1, BARD1, BIRC3, BLM, BRAF, BRCA1, BRCA2, BRIP1, BTK, CBL, CCND1, CCND2, CCND3, CCNE1, CD28, CDH1, CDK12, CDK2, CDK4, CDK6, CDK8, CDKN1A, CDKN1B, CDKN2A, CDKN2B, CHEK1, CHEK2, CIC, CREBBP, CRKL, CSF1R, CSF3R, CTNNB1, DAXX, DDR2, DDX3X, DHX15, DICER, DNMT3A, DOT1L, EED, EGFR, EPHA3, ERBB2, ERBB3, ERBB4, ERCC2, ERRFI1, ESR1, ETNK1, ETV6, EZH2, FAM175A, FANCA, FANCC, FANCD2, FANCI, FANCL, FBXW7, FGF19, FGF3, FGFR1, FGFR2, FGFR3, FGFR4, FLT1, FLT3, FOXL2, FUBP1, GATA1, GATA2, GATA3, GNA11, GNAQ, GNAS, H3F3A, HIST1H3B, HIST1H3C, HNF1A, HRAS, IDH1, IDH2, IGF1R, IGF2, JAK1, JAK2, JAK3, KDR, KIT, KLR4, KMT2D, KNSTRN, KRAS, MAGOH, MAP2K1, MAP2K2, MAP2K4, MAPK1, MAPK3, MAX, MCL1, MDM2, MDM4, MED12, MET, MLH1, MPL, MRE11A, MSH2, MSH6, MTOR, MYC, MYCL, MYCN, MYD88, NBN, NF1, NF2, NFE2L2, NFKBIA, NOTCH1, NOTCH2, NOTCH3, NRAS, NTRK1, NTRK2, NTRK3, PALB2, PARP1, PDGFRA, PDGFRB, PHF6, PIK3CA, PIK3CB, PIK3R1, PIK3R2, PLCG2, PMS2, POLD1, POLE, PPARG, PPP2R1A, PTCH1, PTEN, PTPN11, RAC1, RAD21, RAD50, RAD51, RAD51B, RAD51C, RAD51D, RAF1, RB1, RET, RHEB, RHOA, RICTOR, RNF43, ROS1, SDHA, SDHB, SDHC, SDHD, SETBP1, SETD2, SF3B1, SF3B1, SIRT2, SLIT2, SLX4, SMAD4, SMARCA4, SMARCB1, SMC1A, SMC3, SMO, SOX2, SPOP, SRC, SRSF2, STAT3, STAT5B, STK11, SUFU, TERT, TET2, TOP1, TP53, TRAF7, TSC1, TSC2, TYK2, U2AF1, U2AF2, WT1, XPO1, and ZBTB7A.
Copy number alterations (gains or losses) are detected in these genes: AKT1, AKT2, AKT3, ALK, AR, AXL, BRAF, CCND1, CCND2, CCND3, CCNE1, CDK2, CDK4, CDK6, CDKN2A, CDKN2B, EGFR, ERBB2, ESR1, FGF19, FGF3, FGFR1, FGFR2, FGFR3, FGFR4, FLT3, IGF1R, KIT, KRAS, MDM2, MDM4, MET, MYC, MYCL, MYCN, NTRK1, NTRK2, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CB, PPARG, PTEN, RICTOR, SOX2, TERT, TSC1, and TSC2.
Fusion transcripts are detected and reported involving rearrangements of these genes: AKT1, ALK, AXL, CCND1, EGFR, FGFR1, FGFR2, FGFR3, MET, NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, and ROS1.
Test
Limitations:
Testing is not currently validated for microsatellite instability (MSI) or mutation load analyses. Please contact the laboratory director for questions. Testing is not validated for use on plasma for cell-free tumor DNA mutation testing.
Methodology:
Massively parallel Next Generation DNA sequencing by synthesis, Illumina technology
CPT Code:
81445, 81455, 88342, 88341