Lung NSCLC Targeted RNA Fusions with Interpretation
Label Mnemonic: | CTL LUNG |
Epic code: | LAB8567 |
Downtime form: | A-1a Doctor/Provider Orders - Pathology Molecular |
Molecular Pathology
6240 RCP
384-9568
6240 RCP
384-9568
Specimen(s):
Formalin-fixed, paraffin embedded tissue block, or ten unstained 4
micron-thick sections on non-plus slides and one H&E stained section
Rejection Criteria:
Specimens that have been decalcified will not be accepted.
Turn Around
Time:
10-14 days
Reference Range:
Fusion or mutation not detected
Comments:
Tissue sections are reviewed by a pathologist. RNA is isolated from
the selected area of the sample. Anchored multiplex PCR for targeted
next-generation sequencing is performed. The sequenced sample is a
reverse transcription PCR-amplified fragment library in which each
sample is uniquely identified by ligation of a short oligonucleotide
barcode. The resultant sequence identifies the exons of the fusion
transcript arising from the specific gene target and the partner
gene.
The panel targets gene rearrangements of these gene targets: AKT1, ALK, AXL, BRAF, CCND1, EGFR (vIII), FGFR1, FGFR2, FGFR3, MET (exon 14 skip), NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, ROS, and THADA.
The panel targets gene rearrangements of these gene targets: AKT1, ALK, AXL, BRAF, CCND1, EGFR (vIII), FGFR1, FGFR2, FGFR3, MET (exon 14 skip), NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, ROS, and THADA.
Methodology:
Massively parallel next generation sequencing on RNA
CPT Code:
81445