Lung NSCLC Targeted RNA Fusions with Interpretation
Label Mnemonic: CTL LUNG
Epic Lab Code: LAB8567
Downtime Form: A-1a Molecular Pathology/Diagnostics Laboratory Requisition
Molecular Pathology
6004 BT GH
384-9568
Specimen(s):
Formalin-fixed, paraffin embedded tissue block, or ten unstained 4 micron-thick sections on non-plus slides and one H&E stained section
Rejection Criteria:
Specimens that have been decalcified will not be accepted.
Turn Around Time:
10-14 days
Reference Range:
Fusion or mutation not detected
Comments:
Tissue sections are reviewed by a pathologist. RNA is isolated from the selected area of the sample. Anchored multiplex PCR for targeted next-generation sequencing is performed. The sequenced sample is a reverse transcription PCR-amplified fragment library in which each sample is uniquely identified by ligation of a short oligonucleotide barcode. The resultant sequence identifies the exons of the fusion transcript arising from the specific gene target and the partner gene.

The panel targets gene rearrangements of these gene targets: AKT1, ALK, AXL, BRAF, CONDI, EGFR (vIII), FGFR1, FGFR2, FGFR3, MET (exon 14 skip), NRG1, NTRK1, NTRK2, NTRK3, PPARG, RAF1, RET, ROS, and THADA.
Methodology:
Massively parallel next generation sequencing on RNA
CPT Code:
81445