Herpes Virus 8 (HHV-8) PCR Non-Blood
Label Mnemonic: HHV8O
Epic Lab Code: LAB8109
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Various Sample Types
Collection Medium:
Sterile container
Minimum:
Bronchial Lavage/Bronchial Wash: 2 mL, collected in sterile, screw-cap tube.

Tissue: Place a minimum of 5 mg fresh tissue (approximately 1/2 of a pencil eraser) in sterile, screw top container. Do not add water, saline or other fluid media to container. Store frozen. Formalin fixed, paraffin embedded tissue will not be accepted.

CSF: 2 mL specimens. Place in a sterile, screw top container.

Trach Aspirate/Tracheal Wash: 2 mL collected in a sterile, screw top tube or container.

Call Mailouts at 356-8593 for details.
Rejection Criteria:
Whole blood
Delivery Instructions:
Deliver to laboratory immediately after collection.
Turn Around Time:
24 hours upon receipt in reference laboratory
Reference Range:
Expected value is not detected
Comments:
HHV-8 is the etiologic agent of Kaposi's Sarcoma (KS). Numerous studies have documented a higher risk of developing KS among HIV patients, organ transplant patients, and other immunocompromised individuals. Quantitative HHV-8 DNA PCR can be used to document the presence of the virus as well as track the course of infection.

The primers and probes used in this assay are specific for known strains of HHV-8 based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, JCV, parvovirus B19, SV-40, and VZV.
Methodology:
Quantitative Real-Time PCR
CPT Code:
87799
 
See Additional Information:
Specimens Requiring Immediate Delivery