Paraneoplastic Autoantibodies

Label Mnemonic:	NEOPLAS
Epic code:	LAB7735
Downtime form:	A-1a Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Commercial Mailout Laboratory
6240-8 RCP
356-8593

Specimen(s):

Serum

Collection Medium:

Red top tube 5 mL (Clot Activator)

Minimum:

2 mL serum in red top tube

Rejection Criteria:

Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Turn Around Time:

1-9 days upon receipt at reference laboratory

Reference Range:

Components                                       Reference Interval
Purkinje Cell/Neuronal Nuclear IgG Scm           None Detected

Neuronal Nuclear Antibody (ANNA)                 Less than 1:10
 IFA Titer, IgG

Purkinje Cell Antibody, Titer                    Less than 1:10 

Neuronal Nuclear Antibodies (Hu, Ri, Yo,
  Tr/DNER) IgG by Immunoblot, Serum 
    Component                                    Reference Range
  Neuronal Nuclear Ab (Hu) IgG, IB, Serum           Negative
  Neuronal Nuclear Ab (Ri) IgG, IB, Serum           Negative
  Neuronal Nuclear Ab (Yo) IgG, IB, Serum           Negative
  Neuronal Nuclear Ab (Tr/DNER) IgG, IB             Negative

Comments:

Purkinje Cell (PCCA) antibody and Neuronal Nuclear (ANNA) antibody IgG are screened by IFA. If the IFA screen is indeterminate, then a Neuronal Nuclear Antibodies (Hu, Ri, Yo, and Tr/DNER) IgG by Immunoblot will be added. If the IFA screen is positive at 1:10 or greater, then a PCCA/ANNA antibodies titer and Neuronal Nuclear Antibodies (Hu, Ri, Yo, and Tr/DNER) IgG by Immunoblot will be added. Additional charges apply.

Methodology:

Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Immunoblot

CPT Code:

86255; if reflexed add 84182 x4 and/or 86256