Hepatitis E Virus PCR
Microbiology/Molecular Infectious Disease
Whole Blood, Plasma
ONE 6 mL whole blood in a Pink top tube or TWO 3 mL whole blood in
lavender top tubes. HEV molecular testing cannot be added onto a
previously opened Vacutainer® tube. Testing
requires a dedicated collection tube.
Deliver to laboratory immediately after collection.
Record draw time.
All collection tubes need to be processed within six hours of
collection. If specimens cannot reach the laboratory at University of
Iowa Hospitals and Clinics within the six hours, process as follows:
Pink or lavender top tubes:
Centrifuge for 20 minutes at 800-1600 x g. Aseptically enter the tube
and aliquot into sterile tubes. Transport to the laboratory on ice
within 24 hours.
0800-1700 Testing offered once per week
excluding weekends and University holidays.
Results Reported: Negative, Positive, Indeterminate, Insufficient.
The performance characteristics of this test were determined by the
University of Iowa Microbiology and Molecular Pathology Laboratory. It
has not been cleared or approved by the U.S. Food and Drug
Administration (FDA). The FDA has determined that such clearance or
approval is not necessary. This test is for clinical purposes. It
should not be regarded as investigational or for research. The
laboratory is certified under the Clinical Laboratory Improvement
Amendments of 1988 (CLIA) as qualified to perform high complexity
clinical laboratory testing.
Real-Time reverse transcription Polymerase Chain Reaction (RT-PCR)