Herpes Simplex Virus PCR, Other Fluids
Label Mnemonic: HSVPR
Epic Lab Code: LAB7468
Downtime Form: A-1a Miscellaneous Request
Commercial Mail-out Laboratory
5231 RCP
356-8593
Specimen(s):
Amniotic, Vitreous, Ocular or Aqueous Fluid
Collection Medium:
Sterile container
Minimum:
Preferred Minimum: 1.0 mL fluid
Absolute Minimum: 0.3 mL fluid

Collect in sterile container: Submit 0.5 mL amniotic fluid. If submitting one of the following vitreous/ocular/or aqueous fluid, collect 0.5 mL if ordering one laboratory test. If multiple tests ordered, collect 1.0 mL of this fluid.
(This amount of sample will perform from 1 up to 4 viral tests).
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection period is completed.
Turn Around Time:
24 hours upon receipt at reference laboratory
Reference Range:
Negative
Interpretive Data:
This is a qualitative assay; results are reported either as negative or positive for HSV type 1 or HSV type 2, or HSV indeterminate.

Detection of HSV DNA in clinical specimens supports the clinical diagnosis of infection due to the virus.
Comments:
Useful for an aid in the rapid diagnosis of herpes simplex virus (HSV) infections.

Cautions: A negative result does not eliminate the possibility of herpes simplex virus (HSV) infection. HSV DNA may not be detectable in the early acute stages of the central nervous system disease. In addition, in some cases, after initial detection (positive result), HSV DNA may only be present in cerebrospinal fluid (CSF) for 3 to 4 weeks after initial presentation of symptoms. DNA levels may fall to undetectable with time.

Although the reference range is typically "negative" for this assay, this assay may detect viral shedding in asymptomatic individuals. This may be especially relevant when dermal or genital sites are tested, since intermittent shedding without noticeable lesions has been described. This assay is only to be used for patients with a clinical history and symptoms consistent with HSV infection, and must be interpreted in the context of the clinical picture. This test should not be used to screen asymptomatic patients.
Methodology:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
CPT Code:
87529
 
See also:
Cytomegalovirus by PCR, Other Fluids, Vitreous, Ocular or Aqueous Fluid
Toxoplasma gondii PCR, Other Fluids, Amniotic, Vitreous, Ocular or Aqueous Fluid
Varicella-Zoster Virus PCR, Other Fluids, Amniotic, Vitreous, Ocular or Aqueous Fluid