Lymphocyte Transformation, Antigen
Epic Lab Code: SPL4
Downtime Form: A-1a Miscellaneous Request
VA Diagnostic Immunology Lab
Veterans Affairs Hospital
Rm. 223, Bldg. 41
319-338-0581, EXT 7550
dial 158 from UIHC
Specimen(s):
Whole Blood
Specimen Instructions:
Call VA Diagnostic Immunology Lab as soon as sample is drawn
(319-338-0581) ext. 7550.

Blood cannot be held longer than 24 hours before processing.

ATTENTION: For samples drawn in Medicine Specialty Clinics and Pediatric Specialty Clinics place in VA DIAG. IMM (Ballas) box.

For samples drawn on the floor please call the lab for pick up
(319-338-0581) ext. 7550.
Minimum:
10 mL; sodium heparin green top. Do not use a needle smaller than 21 gauge.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Do not centrifuge.
Reference Range:
Internal control and normal range reported with each sample. Responses to recall antigens assesses the function of memory CD4 lymphocytes, response to alloantigen assesses the function of virgin CD4 and CD8 lymphocytes.
Comments:
This assay is run Monday through Thursday during working hours and Friday 8 a.m. to noon. This assay is run using viable lymphocytes.

This assay examines the ability of T cells to respond to Diphtheria/Tetanus and Candida recall antigens and to alloantigen. For a global assessment of T lymphocyte function, order 'Lymphocyte Transformation, Spontaneous' and 'Lymphocyte Transformation, Mitogen'.
 
See also:
Lymphocyte Transformation, Mitogen, Whole Blood