Lymphocyte Transformation, Mitogen
Epic Lab Code: SPL3
Downtime Form: A-1a Miscellaneous Request
VA Diagnostic Immunology Lab
Veterans Affairs Hospital
Rm. 223, Bldg. 41
319-338-0581, EXT 7550
dial 158 from UIHC
Specimen(s):
Whole Blood
Specimen Instructions:
Call VA Diagnostic Immunology Lab as soon as sample is drawn
(319-338-0581) ext. 7550.

Blood cannot be held longer than 24 hours before processing.

ATTENTION: For samples drawn in Medicine Specialty Clinics and Pediatric Specialty Clinics place in VA DIAG. IMM (Ballas) box.

For samples drawn on the floor please call the lab for pick up
(319-338-0581) ext. 7550.
Minimum:
10 mL; sodium heparin green top. Do not use a needle smaller than 21 gauge.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Do not centrifuge.
Comments:
This assay is run Monday through Thursday during working hours and Friday 8 a.m. to noon. This assay is run using viable lymphocytes.

This assay examines the ability of lymphocytes (T and B) to respond to a polyclonal stimuli (PHA, CON A, PWM, IL-2, and Anti-CD3). A normal response suggests that the patient's T and B lymphocytes have a normal capacity to proliferate upon encountering an appropriate stimulus. For a global assessment of lymphocyte function, order 'Lymphocyte Transformation, Spontaneous' and 'Lymphocyte Transformation, Mitogen' or 'Lymphocyte Transformation, Battery'.