Immunodeficiency Evaluations; Adult and Pediatric
Order form: Epic Beaker AP Order Label
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260
Peripheral Blood
Collection Medium:
Yellow top tube 8.5 mL (ACD solution A)
Alternate Collection Media:
Lavender top tube 3 mL (EDTA)
Adult: 8.5 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a yellow top tube (ACD solution A)
Minimum specimen requirements are cell count dependent.

For absolute quantitative results, a CBC with Automated Differential must also be ordered.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.
Testing Schedule:
0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.
Turn Around Time:
2 days
Reference Range:
Antibodies performed vary with the patient's clinical problem; 
please provide history and lab findings. The pathologist will provide 
an interpretative report.

Adult Immunodeficiency Screening Panel:
CD3, CD4, CD8, CD14, CD19, CD25, CD45, CD56, and CD127

Autoimmune Lymphoproliferative Syndrome (ALPS):
CD2, CD3, CD4, CD8, CD19, CD45, HLA-DR, TCR alpha-beta, and 
TCR gamma-delta
Epic code: LAB8255

Test includes various T and B cell assays, as dictated by the clinical question asked. Include pertinent clinical information on the requisition. Deliver specimen immediately to Specimen Control. Recent corticosteroid or chemotherapy may invalidate result.
Flow Cytometry-Whole Blood Lysis
CPT Code:
88184, 88185 variable - Technical
88187, 88188 or 88189 variable - Professional (varies due to the 
of antibodies performed)
See Additional Information:
Specimens Requiring Immediate Delivery