Immunodeficiency Evaluations; Adult and Pediatric
Epic code: LAB8255
Order form: Epic Beaker AP Order Label
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008 Flow Cytometry Lab: 467-6009 or 467-6010 Tube Station #260
Specimen(s):
Peripheral Blood
Collection Medium:
Yellow top tube 8.5 mL (ACD solution A)
Alternate Collection Media:
Lavender top tube 3 mL (EDTA)
Minimum:
Adult: 8.5 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a yellow top tube (ACD solution A)
Minimum specimen requirements are cell count dependent.

For absolute quantitative results, a CBC with Automated Differential must also be ordered.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.
Testing Schedule:
0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.
Turn Around Time:
2 days
Reference Range:
Antibodies performed vary with the patient's clinical problem; 
please provide history and lab findings. The pathologist will provide 
an interpretative report.

Adult Immunodeficiency Screening Panel:
CD3, CD4, CD8, CD14, CD19, CD25, CD45, CD56, and CD127

Autoimmune Lymphoproliferative Syndrome (ALPS):
CD2, CD3, CD4, CD8, CD19, CD45, HLA-DR, TCR alpha-beta, and 
TCR gamma-delta
Comments:
Test includes various T and B cell assays, as dictated by the clinical question asked. Include pertinent clinical information on the requisition. Deliver specimen immediately to Specimen Control. Recent corticosteroid or chemotherapy may invalidate result.
Methodology:
Flow Cytometry-Whole Blood Lysis
CPT Code:
88184, 88185 variable - Technical
88187, 88188 or 88189 variable - Professional (varies due to the 
number 
of antibodies performed)
 
See Additional Information:
Specimens Requiring Immediate Delivery