Immunodeficiency Evaluations; Adult and Pediatric

Order form:

Epic Beaker AP Order Label

Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260

Specimen(s):

Peripheral Blood

Collection Medium:

Yellow top tube 8.5 mL (ACD solution A)

Alternate Collection Media:

Lavender top tube 3 mL (EDTA)

Minimum:

Adult: 8.5 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a yellow top tube (ACD solution A)
Minimum specimen requirements are cell count dependent.

For absolute quantitative results, a CBC with Automated Differential must also be ordered.

Delivery Instructions:

Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.

Testing Schedule:

0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.

Turn Around Time:

2 days

Reference Range:

Antibodies performed vary with the patient's clinical problem; 
please provide history and lab findings. The pathologist will provide 
an interpretative report.

Adult Immunodeficiency Screening Panel:
CD3, CD4, CD8, CD14, CD19, CD25, CD45, CD56, and CD127

Autoimmune Lymphoproliferative Syndrome (ALPS):
CD2, CD3, CD4, CD8, CD19, CD45, HLA-DR, TCR alpha-beta, and 
TCR gamma-delta

Comments:

Epic code: LAB8255

Test includes various T and B cell assays, as dictated by the clinical question asked. Include pertinent clinical information on the requisition. Deliver specimen immediately to Specimen Control. Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

88184, 88185 variable - Technical
88187, 88188 or 88189 variable - Professional (varies due to the 
number 
of antibodies performed)

See Additional Information:
Specimens Requiring Immediate Delivery