Lymphocyte Subsets
Epic Lab Code: LAB8255
Order Form: Epic Beaker AP Order Label
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260
Specimen(s):
Peripheral Blood
Collection Medium:
Yellow top tube 8.5 mL (ACD solution A)
Alternate Collection Media:
Lavender top tube 3 mL (EDTA)
Minimum:
Adult: 8.5 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a lavender top tube (EDTA)
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.
Testing Schedule:
0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.
Turn Around Time:
2 days
Reference Range:
The pathologist will provide an interpretative report. Antibodies routinely included are: CD3, CD4, CD8, CD16/56, CD19, and CD45. Adult reference ranges for peripheral blood by whole blood lysis method using flow cytometry: B cells (CD19) 6-22% T cells (CD3) 65-85% T helper/induce (CD4+/CD3+) 30-62% T cytotoxic/suppressor (CD8+/CD3+) 13-37% NK cells (CD16+/CD56+/CD3-) 5-31% CD3+/CD16+/CD56+ 0-6% CD4/CD8 ratio 0.7-2.7 Age specific pediatric reference ranges will be provided with the interpretive report.
Methodology:
Flow Cytometry-Whole Blood Lysis
CPT Code:
88184 x1, 88185 x6 Technical, 88187 Professional
 
See Additional Information:
Specimens Requiring Immediate Delivery