Lymphocyte Subsets

Order form:

Epic Beaker AP Order Label

Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009 or 467-6010
Tube Station #260

Specimen(s):

Peripheral Blood

Collection Medium:

Yellow top tube 8.5 mL (ACD solution A)

Alternate Collection Media:

Lavender top tube 3 mL (EDTA)

Minimum:

Adult: 8.5 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a lavender top tube (EDTA)

Delivery Instructions:

Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.

Testing Schedule:

0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.

Turn Around Time:

2 days

Reference Range:

The pathologist will provide an interpretative report.  
Antibodies routinely included are:  CD3, CD4, CD8, CD16/56, CD19, and 
CD45.

Adult reference ranges for peripheral blood by whole blood lysis 
method using flow cytometry:

B cells (CD19)                       6-22%
T cells (CD3)                       65-85%
T helper/induce (CD4+/CD3+)         30-62%
T cytotoxic/suppressor (CD8+/CD3+)  13-37%
NK cells (CD16+/CD56+/CD3-)         5-31%
CD3+/CD16+/CD56+                    0-6%
CD4/CD8 ratio                       0.7-2.7

Age specific pediatric reference ranges will be provided with the 
interpretive report.

Comments:

Epic code: LAB8255

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

88184 x1, 88185 x5 Technical, 88187 Professional

See Additional Information:
Specimens Requiring Immediate Delivery