Amino Acids, Quantitative

Label Mnemonic: AAQTP
Epic code: LAB2578
Downtime form: Doctor/Provider Orders - Pathology Core and Specialty Care Nursery
Commercial Mailout Laboratory
6240-8 RCP
356-8593
Specimen(s):
Plasma
Specimen Instructions:

Patient's age is required on request form for processing. Include family history, clinical condition (asymptomatic or acute episode), diet, and drug therapy information.

Collection Medium:
Green top tube 4 mL (Na Heparin)
Minimum:

Preferred Minimum: 4 mL heparinized plasma in a green-top (heparin) tube from a fasting patient (4 hours or more in infants).
Absolute Minimum: 0.5 mL heparinized plasma

Rejection Criteria:

Thrombin-activated tube is not acceptable.

Testing Schedule:

Monday through Friday

Turn Around Time:

3 days upon receipt at reference laboratory (not reported on Saturday or Sunday)

Reference Range:

Cautions: Reference values are for fasting patients.

Amino acids <24 months 2-17 years > or =18 years

Taurine (Tau)

31-354

32-181

21-123

Asparagine (Asn)

18-94

25-80

23-94

Serine (Ser)

59-224

53-166

55-146

Hydroxyproline (Hyp)

<121

<73

<38

Glycine (Gly)

80-500

80-500

80-500

Glutamine (Gln)

356-857

353-790

447-774

Aspartic Acid (Asp)

<48

<17

<13

Ethanolamine (EtN)

<70

<30

<20

Histidine (His)

46-147

56-119

61-120

Threonine (Thr)

49-358

48-205

73-325

Citrulline (Cit)

8-42

12-44

18-57

Sarcosine (Sar)

<20

<20

<20

b-Alanine (bAla)

<36

<36

<36

Alanine (Ala)

139-474

144-557

200-579

Glutamic Acid (Glu)

28-376

16-182

13-148

1-Methylhistidine (1MHis)

<12

<12

<12

3-Methylhistidine (3MHis)

<11

<30

<35

Argininosuccinic Acid (Asa)

<5

<5

<5

Homocitruline (Hcit)

<5

<2

<2

Arginine (Arg)

28-164

28-156

45-144

a-Aminoadipic Acid (Aad)

<4

<4

<4

g-Amino-n-butyric Acid (GABA)

<4

<4

<4

b-Aminoisobutyric Acid (bAib)

<9

<5

<5

a-Amino-n-butyric Acid (Abu)

<40

<40

<40

Hydroxylysine (Hyl)

<4

<4

<4

Proline (Pro)

102-342

99-389

107-383

Ornithine (Orn)

32-171

32-148

39-154

Cystathionine (Cth)

<4

<4

<4

Cystine (Cys)

6-131

3-151

8-310

Lysine (Lys)

83-304

61-291

105-335

Methionine (Met)

12-57

13-41

13-40

Valine (Val)

94-382

111-367

134-357

Tyrosine (Tyr)

27-188

36-133

36-113

Isoleucine (IIe)

23-149

26-150

29-153

Leucine (Leu)

59-213

51-216

79-217

Phenylalanine (Phe)

36-105

38-116

45-106

Tryptophan (Trp)

12-103

21-114

21-108

Alloisoleucine (Allolle)

<5

<5

<5

 

Interpretive Data:

When abnormal results are detected, a detailed interpretation is given, including an overview of the results and of their significance, a correlation to available clinical information, elements of differential diagnosis, recommendations for additional biochemical testing, and in vitro confirmatory studies (enzyme assay, molecular analysis), name and phone number of key contacts who may provide these studies at the reference laboratory or elsewhere, and a phone number to reach one of the laboratory directors in case the referring physician has additional questions.

Comments:

Useful for:
Evaluating patients with possible inborn errors of metabolism.

May aid in evaluation of endocrine disorders, liver diseases, muscle diseases, neoplastic diseases, neurological disorders, nutritional disturbances, renal failure, and burns.

Test Limitations:

Biochemical test results depend in part on the clinical and dietary status at the time of specimen collection. A normal or non-diagnostic test result does not rule out the possibility of an underlying metabolic disorder, including that for which the test was requested.

Methodology:

Quantitative Analysis by Liquid Chromatography-Tandem Mass Spectometry (LC-MS/MS)

Includes quantitation of the following amino acids: taurine, threonine, serine, asparagine, glutamic acid, glutamine, proline, alanine, citrulline, alpha-amino-n-butyric acid, valine, cystine, methionine, isoleucine, leucine, tyrosine, phenylalanine, beta-alanine, ornithine, lysine, histidine, argininosuccinic acid, allo-isoleucine, arginine, phosphoserine, phosphoethanolamine, hydroxyproline, glycine, aspartic acid, ethanolamine, sarcosine, 1-methylhistidine, 3-methylhistidine, carnosine, anserine, homocitruline, alpha-aminoadipic acid, gamma- amino- n-butyric acid, beta-aminoisobutyric acid, hydroxylysine, cystathionine, and tryptophan.

CPT Code:
82139
 
See Additional Information:
Fasting Specimen Requirements