Immunofixation Electrophoresis

Label Mnemonic:	SIFE
Epic code:	LAB156
Downtime form:	Doctor/Provider Orders - Pathology Core and Specialty Care Nursery

Chemistry
6240 RCP
356-3527

Specimen(s):

Serum

Collection Medium:

Red top tube 5 mL (Clot Activator)

Minimum:

Adult - 2 mL; red top tube
Pediatric - 2 mL; red top tube

Testing Schedule:

Monday - Friday

Turn Around Time:

48 hours

Reference Range:

Negative for monoclonal proteins. The serum IFE report will include a descriptive report of abnormalities, if present.

Comments:

Serum immunofixation electrophoresis methodology switched from traditional gel electrophoresis to capillary electrophoresis on September 24, 2012. Technically, the method used in capillary electrophoresis to identify monoclonal proteins is known as immunotyping. This methodology can resolve IgA, IgG, and IgM heavy chains, as well as kappa and lambda light chains. Monoclonal gammopathies involving IgD and IgE are rare but can occur. Contact pathology resident at page #3725 if additional workup may be needed for workup of suspected IgD or IgE gammopathy.

Methodology:

Capillary electrophoresis with immunotyping

CPT Code:

86334