Immunofixation Electrophoresis
Label Mnemonic: SIFE
Epic Lab Code: LAB156
Downtime Form: A-1a General Laboratory Requisition
Chemistry
6240 RCP
356-3527
Specimen(s):
Serum
Collection Medium:
Red top tube 5 mL (Clot Activator)
Minimum:
Adult - 2 mL; red top tube
Pediatric - 2 mL; red top tube
Testing Schedule:
Monday - Friday
Turn Around Time:
48 hours
Reference Range:
Negative for monoclonal proteins. The serum IFE report will include a descriptive report of abnormalities, if present.
Comments:
Serum immunofixation electrophoresis methodology switched from traditional gel electrophoresis to capillary electrophoresis on September 24, 2012. Technically, the method used in capillary electrophoresis to identify monoclonal proteins is known as immunotyping. This methodology can resolve IgA, IgG, and IgM heavy chains, as well as kappa and lambda light chains. Monoclonal gammopathies involving IgD and IgE are rare but can occur. Contact pathology resident at page #3725 if additional workup may be needed for workup of suspected IgD or IgE gammopathy.
Methodology:
Capillary electrophoresis with immunotyping
CPT Code:
86334