Hepatitis C Virus; Quantitative PCR
Label Mnemonic: HCVQNT
Epic Lab Code: LAB2465
Downtime Form: A-1a Clinical Microbiology Laboratory Requisition
Microbiology/Molecular Infectious Disease
BT 6004
356-2591
Specimen(s):
Plasma
Specimen Instructions:
Record draw time. All collection tubes need to be processed within 24 hours of collection. If specimens cannot reach the Microbiology laboratory at University of Iowa Hospitals and Clinics within the 24 hours, process as follows: Centrifuge for 20 minutes at 800-1600 x g. Aseptically enter the tube and aliquot into sterile tubes. Transport to the laboratory on ice within 24 hours.
Collection Medium:
Pink top tube 6 mL (K2-EDTA)
Minimum:
One full pink top tube (EDTA). Note: If HCV Genotype is also requested, please obtain a second full pink top tube. Sample tube must remain sterile. HCV molecular testing cannot be added onto a previously opened Vacutainer® tube. Testing requires a dedicated collection tube. All collection tubes need to be processed within six hours of collection.
Delivery Instructions:
Deliver to laboratory immediately after collection.
Testing Schedule:
Twice per week
Turn Around Time:
1 week
Reference Range:
Negative Analytical range in log10 values: Log IU (15-100,000,000 IU/mL non-log transformed values) Positive results less than 15 IU/mL will be reported as "POS <1.18 LOG IU", "POS <15 IU/mL" and negative results will be reported as "Not detected".
Methodology:
PCR amplification; Cobas HCV Test (Roche Diagnostics, Inc.)
CPT Code:
87522