Hepatitis C Virus; Quantitative PCR

Label Mnemonic:	HCVQNT
Epic code:	LAB2465
Downtime form:	A-1a Doctor-Provider Orders - Pathology Microbiology

Microbiology/Molecular Infectious Disease
BT 6004
356-2591

Specimen(s):

Plasma

Specimen Instructions:

Record draw time.

All collection tubes need to be processed within 24 hours of 
collection.  If specimens cannot reach the Microbiology laboratory at 
University of Iowa Hospitals and Clinics within the 24 hours, process 
as follows:

Centrifuge for 20 minutes at 800-1600 x g.  Aseptically enter the tube
and aliquot into sterile tubes.  Transport to the laboratory on ice 
within 24 hours.

Collection Medium:

Pink top tube 6 mL (K2-EDTA)

Minimum:

One full pink top tube (EDTA). Note: If HCV Genotype is also requested, please obtain a second full pink top tube. Sample tube must remain sterile. HCV molecular testing cannot be added onto a previously opened Vacutainer^® tube. Testing requires a dedicated collection tube. All collection tubes need to be processed within six hours of collection.

Delivery Instructions:

Deliver to laboratory immediately after collection.

Testing Schedule:

Twice per week

Turn Around Time:

1 week

Reference Range:

Negative

Analytical range in log10 values:
  Log IU (15-100,000,000 IU/mL non-log transformed values)

Positive results less than 15 IU/mL will be reported as "POS <1.18 LOG 
IU", "POS <15 IU/mL" and negative results will be reported as "Not 
detected".

Methodology:

PCR amplification; Cobas HCV Test (Roche Diagnostics, Inc.)

CPT Code:

87522

See Additional Information:
Conversion of Log Value to Integer Value Calculator
Specimens Requiring Immediate Delivery