Enoxaparin Assay (Anti Xa)
Label Mnemonic: ENOX
Epic code: LAB316
Order form: Laboratory Requisition
Supply order: Supply Order Form
Billing: Billing Policies
CPT code: 85520
Specimen(s):
Citrated Plasma More information
Collection Medium:
Light Blue top tube 1.8 mL (Na Citrate)
Minimum:
Full draw; 1.8 mL in light blue-top (3.2% sodium citrate) tube. Mix well.
Testing Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around Time:
2 hours (upon receipt in laboratory)
Reference Range:
The therapeutic range for low molecular weight heparins (LMWH) varies with the type and manufacturer, but for adults is typically 0.4 – 1.1 U/mL. For adults, the target range for VTE prophylaxis is 0.2 – 0.4 U/mL.
Comments:
Patient medications must be recorded on the laboratory requisition.

Double-centrifuged specimens are critical for accurate results as platelet contamination may cause spurious results.

Instrumentation changed from BCS-XP to CS-5100 on 12/16/2019.
Methodology:
Anti Xa activity by chromogenic substrate without antithrombin supplementation.

Assay updated to Siemens Innovance reagent on 03/05/19.
Instructions:
0.5 mL platelet poor plasma needed for testing.
Sample Processing:
Centrifuge within four hours of draw time.
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells.
Separate plasma into plastic container and cap (glass is not acceptable).
Centrifuge a second time to ensure platelet poor plasma and separate plasma into a new, labeled plastic tube, cap.
Sample Storage:
Freeze specimen immediately.
Transport Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Place dry ice on top of specimen in Styrofoam container to maintain frozen temperature.
CPT Code:
85520