Phlebotomy Tubes and Order of Draw

 1.
Blood Cultures

 2.
Discard tube
Discard tube
3 mL "no additive" must be used ONLY if drawing a citrate (blue) tube
with a tube holder and butterfly needle. Used only to remove air from tubing.

 3.

Light Blue 1.8 mL
Light Blue top tube
1.8 mL (Na Citrate)


Light Blue 2.7 mL
Light Blue top tube
2.7 mL (Na Citrate)

 4.
Red top tube
Red top tube
5 mL (Clot Activator)

 5.
Light Green top tube
Light Green top tube
4.5 mL (gel Lithium Heparin) (Plasma Separator Tube - PST)

 6.

Green top tube 4 mL
Green top tube
4 mL (only Na Heparin available at
University of Iowa Hospitals and Clinics


Green top tube 10 mL
Green top tube
10 mL (only Na Heparin available at
University of Iowa Hospitals and Clinics

 7.

Lavender top tube
Lavender top tube
3 mL (K2-EDTA)


Pink top tube
Pink top tube
6 mL (K2-EDTA)

 8.
Gray top tube
Gray top tube
6 mL (Na Flouride)

 9.
Yellow top tube
Yellow top tube
8.5 mL (ACD solution A)

10.
Dark Blue top tube (FDP)
Dark Blue top tube
2 mL Fibrin Degradation Product (FDP) tube; soy trypsin or soy trypsin inhibitor.


Draw Royal Blue top tubes in the order indicated by the additive.


Dark Blue top tube 7 mL
7 mL (No additive) - Red strip on label - Draw as #4


Dark Blue top tube 6 mL
6 mL (K2-EDTA) - Blue strip on label - Draw as #7