The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Epstein-Barr Virus by Quantitative PCR
Order Code: EBVQNTPCR
Epic Lab Code: LAB4804
Order Form: A-1a Miscellaneous Request or Epic Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen:
Serum, Plasma or CSF
Collection Medium:
Lavender top tube (EDTA)
Alternate
Collection Media:
Pink top tube (EDTA sprayed), CSF container
Minimum:
1 mL serum, plasma, or CSF, in a sterile container.

Absolute Adult Minimum:  0.25 mL serum, plasma, or CSF, in a sterile 
container.

Pediatric Minimum:  0.25 mL serum, plasma, or CSF, in a sterile 
container.
Rejection Criteria:
Whole blood, heparinzed specimens
Delivery Instructions:
Submit specimen to laboratory as soon as possible after collection.
Analytic Time:
1 week
Reference Range:
Not detected.
Interpretive Data:
The quantitative range of this assay is 2.6-7.6 log copies/mL 
(390-39,000,000 copies/mL)

A negative result (less than 2.6 log copies/mL or less than 390 
copies/mL) does not rule out the presence of PCR inhibitors in the 
patient specimen, or EBV DNA nucleic acid in concentrations below the 
level of detection of the assay. Inhibition may also lead to 
underestimation of viral quantitation.

No international standard is currently available for calibration of 
this assay. Caution should be taken when interpreting results generated 
by different assay methodologies.
Methodology:
Polymerase Chain Reaction
CPT Code:
87799

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Updated: 11/17/2008

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.