The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Testosterone, Free and Total, Females or Children
Order Code: FTSTFC
Epic Lab Code: LAB4141
Order Form: A-1a Miscellaneous Request or Epic Req
  Commercial "Mail-out" Laboratory
6240 RCP
356-3527
Specimen:
Serum
Collection Medium:
Red top tube
Alternate
Collection Media:
Light Green top tube (Lithium Heparin), Lavender top tube (EDTA), Pink 
top tube (EDTA sprayed), Green top tube (Na Heparin)
Minimum:
Preferred minimum: 1 mL serum or lithium heparin plasma

Absolute minimum: 0.2 mL serum or lithium heparin plasma
Rejection Criteria:
EDTA plasma
Specimen
Instructions:
Specimen should be collected between 6:00 - 10:00 a.m.
Analytic Time:
5 days upon receipt at reference laboratory
Reference Range:
Testosterone, Females or Children
Female - Serum Total Testosterone, Determined by LC-MS/MS
Premature (26-28 weeks): 5-16 ng/dL
Premature (31-35 weeks): 5-22 ng/dL
Newborn:                20-64 ng/dL
1-7 months: Levels decrease during the first month to less than
            10 ng/dL and remain at this level until puberty.
7-9 years:     Less than 15 ng/dL
10-11 years:   2-42 ng/dL
12-13 years:   6-64 ng/dL
14-15 years:   9-49 ng/dL
16-17 years:   8-63 ng/dL
18-30 years:  11-59 ng/dL
31-40 years:  11-56 ng/dL
41-51 years:   9-55 ng/dL
Postmenopausal:    6-25 ng/dL
Tanner Stage I:    Less than 17 ng/dL
Tanner Stage II:   4-39 ng/dL
Tanner Stage III: 10-60 ng/dL
Tanner Stage IV:   8-63 ng/dL
Tanner Stage V:   10-60 ng/dL

Male - Serum Total Testosterone, Determined by LC-MS/MS
Premature (26-28 weeks): 59-125 ng/dL
Premature (31-35 weeks): 37-198 ng/dL
Newborn:                 75-400 ng/dL
1-7 months: Levels decrease rapidly the first week to 20-50 ng/dL,
            and then increase to 60-400 ng/dL between 20-60 days.
            Levels then decline to prepubertal range levels of
            3-10 ng/dL by seven months.
7-9 years:     Less than 9 ng/dL
10-11 years:   2-57 ng/dL
12-13 years:   7-747 ng/dL
14-15 years:  33-585 ng/dL
16-17 years: 185-886 ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 350-890 ng/dL
60 years and older:  300-720 ng/dL
Tanner Stage I:    Less than 20 ng/dL
Tanner Stage II:   2-149 ng/dL
Tanner Stage III:  7-762 ng/dL
Tanner Stage IV: 164-854 ng/dL
Tanner Stage V:   94-783 ng/dL

Sex Hormone Binding Globulin
Age                      Male                       Female
1-30 days:           13-85  nmol/L              14-60  nmol/L
31-364 days:         70-250 nmol/L              60-215 nmol/L
1-3 years:           50-180 nmol/L              60-190 nmol/L
4-6 years:           45-175 nmol/L              55-170 nmol/L
7-9 years:           28-190 nmol/L              35-170 nmol/L
10-12 years:         23-160 nmol/L              17-155 nmol/L
13-15 years:         13-140 nmol/L              11-120 nmol/L
16-17 years:         10-60  nmol/L              19-145 nmol/L
18 years and older:  11-80  nmol/L              30-135 nmol/L
Tanner Stage I:      26-286 nmol/L              30-173 nmol/L
Tanner Stage II:     22-169 nmol/L              16-127 nmol/L
Tanner Stage III:    13-104 nmol/L              12-98  nmol/L
Tanner Stage IV:     11-60  nmol/L              14-151 nmol/L
Tanner Stage V:      11-71  nmol/L              23-165 nmol/L
Interpretive Data:
The concentration of free testosterone is derived from a mathematical 
expression based on the constant for the binding of testosterone to sex 
hormone binding globulin.
Comments:
This test is suggested for women and children due to an improved 
sensitivity of testosterone by LC-MS/MS.
Methodology:
High Performance Liquid Chromatography/Tandem Mass 
Spectrometry/Electrochemiluminescent Immunoassay
The concentration of free testosterone is derived from a mathematical 
expression based on the constant for the binding of testosterone to sex 
hormone binding globulin.
CPT Code:
84403 Testosterone, 84270 Sex Hormone Binding Globulin
 
See also:
Sex Hormone Binding Globulin, Serum or Plasma
Testosterone, Free and Total, Adult Male, Serum
Testosterone, Total, Pediatric, Serum
Testosterone, Total, Plasma

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Updated: 11/13/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.