Specimen

Serum

Collection Medium:

Minimum:

Preferred minimum: 1 mL serum collected from 3 mL whole blood in a red 
top tube

Absolute minimum: 0.5 mL serum collected from 1.5 mL whole blood in a 
red top tube  - This volume does not permit repeat analysis

Delivery Instructions:

Deliver to laboratory within 1 hour of collection.

Specimen
Instructions:

Indicate age and sex on requisition.

Analytic Time:

1 week

Reference Range:

                        Male Range(ng/dL)     Female Range(ng/dL)
PREMATURE INFANTS:
   26-28 Weeks, Day 4        59-125                  5-16
   31-35 Weeks, Day 4        37-198                  5-22

FULL-TERM INFANTS:        Range (ng/dL)
   Newborns 1-7 Months:      75-400                 20-64

   Male:  Levels decrease rapidly the first week to 20-50 ng/dL,
   then increase to 60-400 ng/dL (Mean = 190) between 20-60 days.
   Levels then decline to prepubertal range by <3-10 by seven months.

   Female:  Levels decrease during the first month to <10 ng/dL
   and remain there until puberty.

PREPUBERTAL CHILDREN:
   1-10 Years                <3-10

PUBERTY:              Male                           Female
Tanner     Age        Range      Mean        Age     Range     Mean
Stage    (years)     (ng/dL)   (ng/dL)     (years)  (ng/dL)   (ng/dL)
  1        <9.8      <3-10       4.9        <9.2     <3-10      4.9
  2      9.8-14.5    18-150       42      9.2-13.7    7-28       18
  3     10.7-15.4   100-320      190     10.0-14.4   15-35       25
  4     11.8-16.2   200-620      372     10.7-15.6   13-32       22
  5     12.8-17.3   350-970      546     11.8-18.6   20-38       28

ADULTS 20-50 Years:       Range (ng/dL)
   Male                     350-1030
   Female
     Premenopausal:          10-55
     Postmenopausal:          7-40

Comments:

Includes total testosterone only.

Methodology:

High Performance Liquid Chromatography (HPLC) Tandem Mass Spectrometry

CPT Code:

84403

See also:
Testosterone, Free and Total, Adult Male, Serum
Testosterone, Total, Plasma