Serum

Red top tube

Preferred Minimum: 1 mL serum in a red top tube

Absolute Minimum: 0.2 mL serum in a red top tube - This volume does not permit repeat analysis

EDTA plasma

Deliver to laboratory within 1 hour of collection.

5 days upon receipt at reference laboratory

Serum Total Testosterone, Determined by LC-MS/MS

Female
Premature (26-28 weeks): 5-16 ng/dL
Premature (31-35 weeks): 5-22 ng/dL
Newborn: 20-64 ng/dL
1-7 months: Levels decrease during the first month to less than 10 ng/dL and remain at this level until puberty.
7-9 years: Less than 15 ng/dL
10-11 years: 2-42 ng/dL
12-13 years: 6-64 ng/dL
14-15 years: 9-49 ng/dL
16-17 years: 8-63 ng/dL
18-30 years: 11-59 ng/dL
31-40 years: 11-56 ng/dL
41-51 years: 9-55 ng/dL
Postmenopausal: 6-25 ng/dL
Tanner Stage I: Less than 17 ng/dL
Tanner Stage II: 4-39 ng/dL
Tanner Stage III: 10-60 ng/dL
Tanner Stage IV: 8-63 ng/dL
Tanner Stage V: 10-60 ng/dL

Male
Premature (26-28 weeks): 59-125 ng/dL
Premature (31-35 weeks): 37-198 ng/dL
Newborn: 75-400 ng/dL
1-7 months: Levels decrease rapidly the first week to 20-50 ng/dL, and then increase to 60-400 ng/dL between 20-60 days. Levels then decline to pre-pubertal range levels of 3-10 ng/dL by seven months.
7-9 years: Less than 9 ng/dL
10-11 years: 2-57 ng/dL
12-13 years: 7-747 ng/dL
14-15 years: 33-585 ng/dL
16-17 years: 185-886 ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL
Tanner Stage I: Less than 20 ng/dL
Tanner Stage II: 2-149 ng/dL
Tanner Stage III: 7-762 ng/dL
Tanner Stage IV: 165-854 ng/dL
Tanner Stage V: 194-783 ng/dL

This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.

High Performance Liquid Chromatography (HPLC)/Tandem Mass Spectrometry

84403

See also:
Testosterone, Free and Total, Adult, Plasma
Testosterone, Total, Plasma