Specimen:

Peripheral Blood or Bone Marrow

Collection Medium:

Minimum:

Adult and Pediatric: Peripheral Blood, 10 mL; yellow top tube (ACD 
solution A)

Rejection Criteria:

Specimens with absolute lymphocyte counts of <100/mm3 will not be
tested.

Delivery Instructions:

Keep at room temperature.  Do not refrigerate.
Deliver specimen immediately to Immunopathology Lab to insure leukocyte 
viability.  Do not deliver to specimen control area.

Testing Schedule:

0800-1630 Monday through Friday.  For additional services, 
contact Clinical Pathology Resident on-call at pager #3404.

Analytic Time:

2 days

Reference Range:

The pathologist will provide an interpretative report.

Antibodies routinely included are:  CD3, CD4, CD8, CD14, CD16+56, CD19, 
CD20 and CD45.

Adult reference ranges for peripheral blood by whole blood lysis method 
using flow cytometry:
                                             Absolute Counts
B cells (CD20)                       6-22%   53-726/mm3
T cells (CD3)                       65-85%  569-2804/mm3
T Cells (CD4)                       34-62%  298-2045/mm3
T cells (CD8)                       14-42%  122-1386/mm3
NK cells (CD16+/CD56+/CD3-)         5-31%   44-1023/mm3
CD4/CD8 ratio                       0.7-2.7

Age specific pediatric reference ranges will be provided with the 
interpretive report.

Comments:

Include pertinent clinical information on the reqisition.

Methodology:

Flow Cytometry-Whole Blood Lysis

CPT Code:

CPT Codes: 88184 x1, 88185 x7 - Technical
           881887 - Professional
          (varies due to the number of antibodies performed)

See Additional Information:
Specimens Requiring Immediate Delivery