Chronic Lymphocytic Leukemia
Order Form: A-1a Immunopathology or Epic Req
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009
Tube Station #260
Specimen:
Various
Collection Medium:
Yellow top tube (ACD solution A)
Alternate Collection Media:
Green top tube 4 mL (Na Heparin), Lavender top tube 3 mL (EDTA)
Minimum:
Adult Peripheral Blood: 10 mL; Bone Marrow: 2-4 mL yellow top tube (ACD solution A) Pediatric Peripheral Blood: 2-5 mL; Bone Marrow: 2-5 mL lavender top tube (EDTA) or green top tube (Na Heparin). Fluids and Tissue: Dispense sample into vial of RPMI-1640 tissue culture media.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.
Testing Schedule:
0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.
Analytic Time:
2 days
Reference Range:
Antibodies routinely included are: CD2, CD3, CD5, CD10, CD11c, CD14, CD19, CD20, CD22, CD23, CD25, CD38, CD45, CD103, CD138, FMC7, Heavy chains, Kappa and Lambda. Antibodies will also identify cases of Prolymphocytic and Hairy Cell Leukemia. If clinical or morphologic features suggest a "T" or "NK" lymphocyte disorder, then the following additional antibody combinations are performed: CD3/CD4/CD8, CD7/CD5/HLA-DR, CD14/CD45, CD25/CD2/CD3, CD16/CD56/CD19, CD57/CD8/CD3, TCR alpha-beta/delta-gamma/CD3. The pathologist will provide an interpretative report.
Comments:
Please state the clinical question to be answered on the requisition. Specimens accepted from Monday 0800 until Friday 1630. Clinical Pathology resident should be contacted if studies are needed emergently at other times.
Methodology:
Flow Cytometry-Whole Blood Lysis
CPT Code:
88184, 88185(x20) technical; 88189 professional; T cell or NK cell disorder: 88184, 88185(x15) technical; 88188 professional
 
See Additional Information:
Specimens Requiring Immediate Delivery