Lymphocyte Subsets
Order Form: A-1a Immunopathology or Epic Req
Flow Cytometry Service
6240 RCP
Flow Cytometry Resident: 467-6008
Flow Cytometry Lab: 467-6009
Tube Station #260
Specimen:
Peripheral Blood
Collection Medium:
Yellow top tube (ACD solution A)
Alternate Collection Media:
Lavender top tube 3 mL (EDTA)
Minimum:
Adult: 10 mL whole blood in a yellow top tube (ACD solution A)
Pediatric: 2 mL whole blood in a lavender top tube (EDTA)

For absolute quantitative results, a CBC with Automated Differential must also be ordered.
Delivery Instructions:
Keep at room temperature. Do not refrigerate. Send to tube station #260 or deliver to Specimen Control, 6240 RCP.
Testing Schedule:
0800-1630 Monday through Friday. For additional services, contact Clinical Pathology Resident on-call at pager #3404.
Analytic Time:
2 days
Reference Range:
The pathologist will provide an interpretative report. Antibodies routinely included are: CD3, CD4, CD8, CD14, CD16/56, CD19, CD20 and CD45. Adult reference ranges for peripheral blood by whole blood lysis method using flow cytometry: Absolute Counts B cells (CD19) 6-22% 53-726/mm3 T cells (CD3) 65-85% 569-2804/mm3 T cells (CD4) 34-62% 298-2045/mm3 T cells (CD8) 14-42% 122-1386/mm3 T helper/induce (CD4+/CD3+) 30-62% 263-2045/mm3 T cytotoxic/suppressor (CD8+/CD3+) 13-37% 114-1221/mm3 NK cells (CD16+/CD56+/CD3-) 5-31% 44-1023/mm3 NK cells (CD56) 5-29% 44-957/mm3 CD4/CD8 ratio 0.9-3.6 Age specific pediatric reference ranges will be provided with the interpretive report.
Comments:
Specimens with absolute lymphocyte counts of <100/mm3 will not be tested.
Test Limitations:
Specimens with absolute lymphocyte counts of <100/mm3 will not be tested.
Methodology:
Flow Cytometry-Whole Blood Lysis
CPT Code:
88184 x1, 88185 x7 Technical, 88187 Professional
 
See Additional Information:
Specimens Requiring Immediate Delivery