The University of Iowa (UIHC)
Department of Pathology
LABORATORY SERVICES HANDBOOK


Hepatitis C Virus RNA by PCR
Order Code: HCVQNT
Epic Lab Code: LAB2465
Order Form: A-1a Molecular Pathology/Diagnostics or Epic Req
  Microbiology
BT 6004
356-2591 (0700-2300)
Bacteriology/Virology Section

356-3527 (2300-0700)
Core Lab
Specimen:
Plasma
Collection Medium:
or and or
Pink top tube (EDTA sprayed) Lavender top tube (EDTA) Lavender top tube (EDTA) White top plasma preparation tube (PPT, EDTA)
Minimum:
6 mL whole blood or 3 mL plasma. If also testing for HIV viral load, 
collect a second Pink top tube (3 mL plasma or 6 mL whole blood).  HCV 
molecular testing cannot be added onto a previously opened vacutainer 
tube.  Testing requires a dedicated collection tube.
Delivery Instructions:
Deliver to laboratory immediately after collection.
Specimen
Instructions:
Record draw time.

All collection tubes need to be processed within six hours of 
collection.  If specimens cannot reach the Microbiology laboratory at 
UIHC with the six hours, process as follows:

Pink or lavender top tubes:
Centrifuge for 20 minutes at 800-1600 x g.  Aseptically enter the tube
and aliquot into sterile RNase free tubes.  Transport to the laboratory 
on ice within 24 hours.

White top tube:
Centrifuge for 10 minutes at 800-1600 x g.  Do not pour off plasma, 
maintain sterility and transport to the laboratory on ice within 24 
hours.
Testing Schedule:
Once per week
Analytic Time:
1 week
Reference Range:
Analytic Measurement Range:  43-69,000,000 IU/mL
Results Reported:  Negative, <43 IU/mL, 43 and greater IU/mL
Methodology:
Homogeneous Polymerase Chain Reaction (real time PCR)
CPT Code:
87522
 
See Additional Information:
Specimens Requiring Immediate Delivery

Alphabetic main page

Updated: 09/29/2009

Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.