Requisition Completion, Specimen Labeling and Phlebotomy Guidelines


All laboratory testing requires the creation and completion of a visit for the date of the sample collection. If laboratory testing is to be completed on the day of the visit, the submitting department is responsible for creating a visit for the checking-in the patient prior to the collection of the laboratory sample. If a sample for laboratory testing is received after a patient visit and sent to the laboratory for testing, the submitting department is responsible for creating a visit for the date that the sample was collected.
  1. Patient Care Areas using Epic order entry: Complete and sign laboratory orders within the Epic clinical information system. [Skip to item #3 below.]


  2. For areas not live on Epic Orders and during Epic down-time: Complete requisition for appropriate laboratory:

  3. All requisitions (both Epic generated and non-electronic) require the date and time of collection and the collector's initials be recorded. Microbiology also requires the collectors title.


  4. Determine correct tube type and blood volume for tests ordered (e.g. anti-coagulant, additive, phlebotomy tubes and order of draw). The correct sequence to draw blood specimens or to transfer from a syringe is:

    >>>Blood cultures, light blue top, red top, light green (PST) green top, lavender top and/or pink, white top, gray top, yellow top, and Fibrin Degradation Products (FDP) top.


  5. NOTE:
    1. If a light blue top tube is drawn with a holder and butterfly needle, prime the tubing with a discard tube before filling the sodium citrate tube (light blue topped).

    2. Specimens for Blood Bank require a specific patient identification and collection process. Refer to "Blood Center Services" additional information.


  6. Identify the patient using two identifiers. On in-patients compare the name and hospital number from the arm band to that on the requisition and labels. If patient is an outpatient, ask them to state their full name and date of birth. If patient is an infant or confused adult, check arm band or ask person accompanying the patient for identification. See Patient Identifiers (UIHC Policy V.35)


  7. After drawing, but before leaving the patient, label the tube(s) with two patient identifiers (PID) labels. Transport to the appropriate laboratory. Consult the Epic requisition or the Pathology Laboratory Services Handbook for appropriate transportation requirements, e.g., "on ice".


  8. Intravenous Fluids: If possible, draw blood from the arm/hand that does NOT have intravenous fluid being administered. Tests results may be altered by dilution and/or content of the intravenous fluid, resulting in misleading laboratory values.


  9. For samples that MUST be collected from an arm with an active I.V., the best option is to collect the sample distal (below) the intravenous site. The infusion must be turned off for a MINIMUM of 2 minutes, before drawing the sample. If the only available site is proximal (above) to the I.V. site, turn off the I.V. for at least 2 minutes and draw from a vein other than the vein with the I.V. line. Record "drawn distal/proximal to I.V." in the patient's chart. Follow the same procedure if a sample must be drawn during blood products transfusion. Best practice is to wait one hour after the transfusion is completed before drawing the sample.

STORES# TUBE, BLOOD COLLECTION SIZE BD#
TUBE TOP COLOR
  CONTACT SPECIMEN CONTROL 6 & 7 ML  
ROYAL BLUE
(EDTA/SERUM)
922749 ACD SOLUTION A 8.5 ML 364606
YELLOW
923258 LITH HEPARIN & GEL 0.6 ML 365958
GREEN
907688 K2 EDTA 3 ML 367856
LAVENDER
924085 NA HEPARIN 4 ML 367871
GREEN
907693 NA HEPARIN 10 ML  
GREEN
907689 CITRATE 1.8 ML 363080
LIGHT BLUE
907690 CITRATE 2.7 ML 363083
LIGHT BLUE
907692 K2 EDTA 6 ML 367899
PINK
907694 NA FLUORIDE 6 ML 367925
GRAY
907695 PST WITH LITHIUM HEPARIN & GEL 4.5 ML 367963
LIGHT GREEN
924088 PLASMA PREPARATION TUBE
K2 EDTA & GEL
5 ML 362788
WHITE
924089 FDP TUBE 2 ML 366478
DARK BLUE
YELLOW LABEL
907752 CLOT ACTIVATOR 5 ML 367814
RED
924265 EDTA .6 ML Sarstedt 15.1671
LAVENDER


The following forms are only used if Epic is unavailable or in select areas where Epic is not live.

Form/Requisition Name Processed Stores Order #
Form A-1a  Blood Center Request 922339
Form 0-9  Bone Marrow Examination Request 922993
Form A-1a  Clinical Microbiology Doctor's Order Form 923618
Form A-1a  Critical Care/Special Care Nurseries Laboratory 923779
Form H-2  Cytology Consult Report 922380
Form A-1a TDA  Doctor's Order Therapeutic Drug Assay Not Available through
Processed Stores
Form A-1a  Immunopathology Laboratory 922943
Form A-1a  Miscellaneous Request 922348
Form A-1a Lab  Pathology Doctor's Order-Lab Test 923533
Form A-1a Lab  Pathology Doctor's Order-Inpatient Service
923766
Form A-1a  Molecular Pathology/Diagnostics Laboratory Not Available through
Processed Stores
Form H-1  Surgical Pathology Consultation Request 922379