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Hematopathology Consultation Service
Nancy Rosenthal, MD and Sergei Syrbu, MD, PhD and Carol Holman, MD, PhD
A national anatomic and clinical pathology reference laboratory
affiliated with UI Health Care

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Flow Cytometry

The Immunopathology Laboratory in the Department of Pathology at the University of Iowa offers a comprehensive diagnostic flow cytometry service. Specimens may be sent for flow cytometric analysis alone or with accompanying tissue for morphologic interpretation. A variety of screening and comprehensive panels are available and are listed below.

Our hematopathologists and immunopathologists are available for consultation on questions related to diagnosis and specimen handling issues.

Turn around time is 2 days with a preliminary result available on the day the specimen is received.

Screening Panel: Leukemia/Lymphoma/MDS
When there is a concern for a hematologic malignancy an initial Immunophenotyping (triage) panel is performed. The panel contains ten selected antibodies most commonly used to detect myeloid/lymphoid blasts and B-cell clonality. If the screen is negative no further testing is required. If an atypical population is identified (myeloid or lymphoid), additional testing will be performed in order to render a final diagnosis and classification.

CD5, CD10, CD19, CD33, CD34, CD45, CD64, CD117, KAPPA, LAMBDA

Chronic Lymphocytic Leukemia/B-Cell Lymphoma
CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD20, CD23, CD25, CD38, CD45, CD (16+56), CD103, CD138, FMC7, KAPPA, LAMBDA

Large Granular Leukemia/or T-Cell Lymphoproliferative Disorder
CD3, CD4, CD5, CD7, CD8, CD19, CD56, CD57, KAPPA, LAMBDA, TCR-alpha, TCR-beta

Plasma Cell Neoplasm
CD19, CD20, CD38, CD45, CD56, CD138, KAPPA, LAMBDA

B-Cell Lymphoma staging
When staging (bone marrow aspirate) the patient with known diagnosis of B-cell lymphoma/leukemia, a limited panel of antibodies is performed.
CD5, CD10, CD19, CD20, CD23, CD45, KAPPA, LAMBDA

T-Cell Lymphoma Staging
CD3, CD4, CD5, CD7, CD8, CD14, CD45, CD56

Paroxysmal Nocturnal Hemoglobinuria

Sezary Syndrome
CD3, CD4, CD7, CD8, CD45

ZAP-70 for CLL only
CD (3+56), CD5, CD19, CD45, cytoplasmic ZAP-70

Lymphocyte Subsets: T, B, and NK Cells
CD3, CD4, CD8, CD (16+56), CD19, CD20, CD45