Hepatitis B Core Antibody, IgM
| Label Mnemonic: | HBCM |
| Epic code: | LAB624 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 86705 |
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL whole blood from light green top tube or TWO
Microtainer® devices for pediatric patients
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
See Interpretive Data
Interpretive Data:
A negative result of <0.90 indicates that IgM anti-HBc antibodies were
not detected in the sample. A negative test result does not exclude
the possibility of exposure to or infection with HBV.
A positive result of >1.10 indicates presumptive evidence of hepatitis B virus infection. IgM anti-HBc antibodies were detected in the sample which suggests either on-going or previous HBV infection.
A positive result of >1.10 indicates presumptive evidence of hepatitis B virus infection. IgM anti-HBc antibodies were detected in the sample which suggests either on-going or previous HBV infection.
Comments:
This assay may be significantly impacted by high-dose biotin (>5 mg
dose) taken within previous 12 hours. High concentrations of biotin
may lead to falsely decreased results. These concentrations may be
found in patients taking over-the-counter supplements with biotin
content much higher than nutritional requirements for biotin.
Specimens should not be collected until at least 12 hours after the
last dose.
Current methods for the detection of IgM anti-HBc may not detect all infected individuals. False negative results may occur due to antibody levels below the detection limit of this assay or if the patient's antibodies do not react with the antigen used in this test. A positive anti-HBc IgM result does not exclude co-infection by another hepatitis virus.
The assay is limited to the detection of IgM anti-HBc in human serum or plasma. It can be used to determine whether a patient has, or has recently had, acute or subclinical hepatitis B infection. Supportive clinical information, including other hepatitis B markers, should also be evaluated. The test cannot determine a patient's immune status to hepatitis B.
Current methods for the detection of IgM anti-HBc may not detect all infected individuals. False negative results may occur due to antibody levels below the detection limit of this assay or if the patient's antibodies do not react with the antigen used in this test. A positive anti-HBc IgM result does not exclude co-infection by another hepatitis virus.
The assay is limited to the detection of IgM anti-HBc in human serum or plasma. It can be used to determine whether a patient has, or has recently had, acute or subclinical hepatitis B infection. Supportive clinical information, including other hepatitis B markers, should also be evaluated. The test cannot determine a patient's immune status to hepatitis B.
Test
Limitations:
The assay is unaffected by icterus < 25 mg/dL, hemolysis (Hb < 2000
mg/dL, or lipemia < 1000 mg/dL.
Methodology:
Electrochemiluminescence Immunoassay
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample
Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
86705
See Additional Information:
Biotin Interference with Immunoassays
Biotin Interference with Immunoassays