Fetal Erythrocyte Quantitation

Label Mnemonic:	FHGB
Epic code:	LAB292
Order form:	Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	88184

Specimen(s):

Maternal Peripheral Blood

Collection Medium:

Lavender top tube 3 mL (EDTA)

Minimum:

1 mL whole blood

Rejection Criteria:

Cord blood samples are NOT accepted.

Testing Schedule:

0800-1630 Monday through Friday.

Turn Around Time:

24 hours (upon receipt in laboratory)

Reference Range:

Reference range is less than 0.3%
Positive specimens reported as percent of maternal cells.

The normal "Hemoglobin F value" for non-pregnant adults is less than
0.1%.

Note: This reference range is established as the level at which 
greater than the usual 300 micrograms dose administered to Rh-negative 
women at delivery is required to prevent sensitization.

                      % HbF by Flow     Total RhoGAM (vials)
                         < 0.30%                 1
                       0.3% - 0.89%              2
                       0.9% - 1.49%              3
                       1.5% - 2.09%              4
                       2.1% - 2.69%              5
                          >=2.7%        Contact DBC Faculty

Comments:

Please identify as MATERNAL or FETAL specimen. Screening test for fetal-maternal bleed.

Call Flow Cytometry (319-467-6010) if testing is required on a fetal sample.

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Immunopathology Requisition before shipping the specimen.

Methodology:

Flow Cytometry

Instructions:

Maintain sample at room temperature; do not incubate or refrigerate.

Optimal results are obtained from a sample within 6 hours of venipuncture. This procedure may obtain results from a sample up to 30 hours from collection.

Sample Processing:

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.

Sample Storage:

Room Temperature.

Transport Instructions:

Place specimen into zip-lock type bag, seal bag.
Place requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.

CPT Code:

88184