Gentamicin Drug Level
| Label Mnemonic: | GENT |
| Epic code: | LAB28 |
| Order form: | Laboratory Requisition |
| Supply order: | Supply Order Form |
| Billing: | Billing Policies |
| CPT code: | 80170 |
Specimen(s):
Plasma
Collection Medium:
 |
| Plasma Separator Tube 4.5 mL |
Alternate
Collection Media:
Call laboratory for additional acceptable specimen collection containers.
Minimum:
3 mL plasma from light green top tube or ONE
Microtainer®
Testing
Schedule:
24 hrs/day, 7 days a week, including holidays.
Turn Around
Time:
1 hour (upon receipt in laboratory)
Reference Range:
Reference range in Epic applies to trough levels when expected value should be <= 1.0 μg/mL. Trough levels should ideally be collected not more than 30 min. before next dose. Different target range would apply to peak levels. Approximate target peak: 5-8 μg/mL. Peak levels: 45-75 min. after I.M. dose, 15-30 min. after I.V. dose. Critical value: >15 μg/mL
Comments:
Clinical staff must draw accurately timed peak and trough specimens.
Reference range changed 12/5/17.
Test
Limitations:
Sample Integrity Confirmation
Icterus: No significant interference from bilirubin up to an I index of 20.
Hemolysis: No significant interference from hemoglobin up to an H index of 2000.
Lipemia: No significant interference from lipemia up to an L index of 1691.
Sisomicin shows significant interference (greater than 50%) with the assay.
The Gentamicin II System Pack assay performance has not been established with body fluids other than human serum and heparin or EDTA plasma.
As with any assay employing mouse antibodies, the possibility exists for interference by human anti-mouse antibodies (HAMA) in the sample, which could cause falsely elevated results.
The incidence of patients having antibodies to E. coli beta-galactosidase is extremely low. However, some samples containing such antibodies can result in artificially high gentamicin results that do not fit the clinical profile. If this occurs, contact Customer Technical Support at 1-800-428-2336 for assistance.
A list of substances known to affect gentamicin levels in vivo is given by Young. No representation is made by Boehringer Mannheim regarding the completeness of this list or the accuracy of the information contained therein.
Icterus: No significant interference from bilirubin up to an I index of 20.
Hemolysis: No significant interference from hemoglobin up to an H index of 2000.
Lipemia: No significant interference from lipemia up to an L index of 1691.
Sisomicin shows significant interference (greater than 50%) with the assay.
The Gentamicin II System Pack assay performance has not been established with body fluids other than human serum and heparin or EDTA plasma.
As with any assay employing mouse antibodies, the possibility exists for interference by human anti-mouse antibodies (HAMA) in the sample, which could cause falsely elevated results.
The incidence of patients having antibodies to E. coli beta-galactosidase is extremely low. However, some samples containing such antibodies can result in artificially high gentamicin results that do not fit the clinical profile. If this occurs, contact Customer Technical Support at 1-800-428-2336 for assistance.
A list of substances known to affect gentamicin levels in vivo is given by Young. No representation is made by Boehringer Mannheim regarding the completeness of this list or the accuracy of the information contained therein.
Methodology:
EIA (Enzymatic Immunoassay)
Sample
Processing:
Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.
Sample
Storage:
Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).
Transport
Instructions:
Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.
CPT Code:
80170
See Additional Information:
Chemistry Critical Lab Values
Chemistry Critical Lab Values