Factor 5 Leiden Mutation & Factor 2 20210G>A Variant with
Interpretation
Label Mnemonic: | PROTPCR |
Epic code: | LAB346 |
Order form: | Molecular Genetics General Consult Requisition |
Supply order: | Supply Order Form |
Billing: | Billing Policies |
CPT code: |
81241 - Leiden Variant Factor 5 81240 - F2 20210G>A Variant Factor 2 |
Specimen(s):
Whole Blood
Collection Medium:
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Pink top tube 6 mL (K2-EDTA) |
Minimum:
6 mL whole blood in pink top tube (EDTA)
Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing.
Testing requires a dedicated collection tube.
Testing on smaller volumes than those requested will be attempted. However, in some cases, small blood volumes may compromise the ability to perform testing.
Testing requires a dedicated collection tube.
Testing Schedule:
Weekly
Turn Around Time:
14 days
Reference Range:
Normal
Comments:
Genomic DNA is PCR amplified and the presence of the Factor 5 Leiden
(individually orderable as LAB9067) and Factor 2 (individually
orderable as LAB9068) mutations associated with venous thrombosis
are assessed simultaneously by gel electrophoresis.
Methodology:
Polymerase Chain Reaction (PCR) - Amplification Refractory Mutation
Systems (ARMS)
Sample Processing:
Do Not Centrifuge.
Label transport tube with two patient identifiers, date and time of collection.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Submit whole blood in original container.
Label transport tube with two patient identifiers, date and time of collection.
Patient's age and sex is required on requisition for processing.
Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Submit whole blood in original container.
Sample Storage:
Room temperature for up to 24 hours, then refrigerate the whole
blood if it is necessary to be held overnight, weekends, or
holidays.
Transport Instructions:
Recommend early AM overnight shipping or equivalent if not on
courier service.
Place requisition into outside pocket of bag.
Ship refrigerated.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.
Place requisition into outside pocket of bag.
Ship refrigerated.
DO NOT FREEZE, protect specimen by wrapping in bubble-wrap or toweling.