Acute Leukemia

Order form:	Comprehensive Hematopathology Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	Technical: 88184 (first antibody), 88185 (each additional antibody); Professional: 88189

Specimen(s):

Peripheral Blood, Bone Marrow, or CSF

Collection Medium:

Yellow top tube 8.5 mL (ACD solution A)

Alternate Collection Media:

Green top tube 4 mL (Na Heparin) or Lavender top tube 3 mL (EDTA)

Minimum:

Adult - Peripheral Blood: 10 mL; Bone Marrow:  2-4 mL

Pediatric - Peripheral Blood:  2-5 mL; Bone Marrow:  2-5 mL

CSF - Volume required is cell count dependent.  Body fluid specimens 
containing small number of leukocytes may not be adequate for 
immunophenotyping. Provide as much CSF specimen as possible in the 
original CSF collection tube(s).

Testing Schedule:

0800-1630 Monday through Friday.

Turn Around Time:

2 days

Reference Range:

Antibodies routinely included are: CD1A, CD2, CD3, CD4, CD5, CD7, 
CD8, CD10, CD11B, CD13, CD14, CD15, CD16, CD19, CD20, CD22, CD33, 
CD34, CD36, CD38, CD45, CD56, CD61, CD64, CD71, CD117, HLA-DR, Kappa, 
Lambda.

Additional antibodies are added as necessary for diagnosis after 
morphologic examination. The pathologist will provide an 
interpretative report.

Comments:

Epic code: LAB8255 for Blood, LAB8284 for Bone Marrow, LAB8254 for Other

For further details, refer to How to Send Flow Cytometry Specimens.

If after hours service is needed, contact UIHC operator at 319-356-1616 and have the Pathology Resident on-call paged.

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Comprehensive Hematopathology Requisition before shipping the specimen. Include pertinent clinical information on the requisition.

Additional antibodies may be necessary for diagnosis.

Test Limitations:

Recent corticosteroid or chemotherapy may invalidate result.

Methodology:

Flow Cytometry-Lysed Whole Blood/Marrow

Sample Processing:

Relevant clinical information must be submitted with specimen in order to provide correct interpretation of test results.
Specimen should be collected and packaged as close to shipping time as possible.

Sample Storage:

Room Temperature.

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place requisition into outside pocket of bag.
Ship at room temperature.
Recommend early AM overnight shipping or equivalent if not on courier service.

CPT Code:

Technical: 88184 (first antibody), 88185 (each additional antibody); Professional: 88189