Cholesterol, High-Density Lipoprotein

Label Mnemonic:	HDLP
Epic code:	LAB101
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	83718

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or 1 Microtainer^Â® for pediatric patients

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

Guidelines from the National Lipid Association and the National Cholesterol Education Program for cardiovascular risk for adults 18 years of age and older:

Males: 40 mg/dL or higher

Females: 50 mg/dL or higher

Comments:

Fasting for at least 8 hours prior to collection is recommended.

Please print, complete and submit the Advance Beneficiary Notice (ABN) along with the Laboratory Requisition before shipping the specimen.

Test Limitations:

Criterion: Recovery within plus or minus 10% of initial value.



Icterus: No significant interference up to an I index of 60.



Hemolysis: No significant interference up to an H index of 1200 

(approximate hemoglobin concentration: 1200 mg/dL).



Lipemia (Intralipid): No significant interference up to an L index of 

2000. There is poor correlation between turbidity and triglycerides 

concentration.



Ascorbic acid: No significant interference up to 50 mg/dL.



In rare cases, elevated immunoglobulin concentrations can cause 

unreliable results.  Abnormal liver function does affect lipid 

metabolism; consequently, HDL and LDL results are of limited 

diagnostic value. In some patients with abnormal liver function, the 

HDL result may significantly differ from the DCM (designated 

comparison method) result.



No interference was found at therapeutic concentrations using common 

drug panels. Statins and fibrates tested at therapeutic concentration 

ranges did not interfere.  N-acetylcysteine at the therapeutic 

concentration when used as an antidote and the acetaminophen 

metabolite N-acetyl-p-benzoquinone imine (NAPQI) independently may 

cause falsely low results.



Venipuncture immediately after or during the administration of 

metamizole may lead to falsely low results.

Methodology:

Enzymatic

Assay updated to Roche HDL Generation 4 on 06/04/18

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.

See Additional Information:
Fasting Specimen Requirements