Chloride

Label Mnemonic:	CL
Epic code:	LAB59
Order form:	Laboratory Requisition
Supply order:	Supply Order Form
Billing:	Billing Policies
CPT code:	82435

Specimen(s):

Plasma

Collection Medium:

Plasma Separator Tube 4.5 mL

Alternate Collection Media:

Call laboratory for additional acceptable specimen collection containers.

Minimum:

3 mL whole blood from light green top tube or 1 Microtainer^® for pediatric patients

Testing Schedule:

24 hrs/day, 7 days a week, including holidays.

Turn Around Time:

1 hour (upon receipt in laboratory)

Reference Range:

95-107 mEq/l

Test Limitations:

Patients over a wide anion gap range demonstrated no significant 
chloride bias from coulometric titrations. Bromide at 0.7 mmol/l gave 
a 
1 mmol/l chloride response (4).  Selectivity of chloride cartridge 
(mmol/l of anion required to increase chloride results by 1 mmol/l) 
(4).

A list of substances and conditions known to affect the level of 
sodium, potassium or chloride in vivo is given by both Young et al (5) 
and Friedman et al (6).  No representation is made by Roche 
Diagnostics 
regarding the completeness of these lists or the accuracy of the 
information contained therein.

Hemolysis: No significant interference up to an H index of 1000.

Icterus: No significant interference up to an I index of 60
(approximate conjugated and unconjugated bilirubin concentration: 60
mg/dL).

Lipemia: No significant interference up an L index of 2000.

References:
(1) Glick MR, Ryder KW, Glick SJ. Interferographs: Users Guide to
    Interferences in Clinical Chemistry Instruments, 2nd ed.
    Indianapolis, IN: Science Enterprises Inc; 1991:35-44.

(2) Tietz NW. Fundamentals of Clinical Chemistry. Philadelphia, Pa:
    3rd ed. WB Saunders Co; 1987:617.

(3) Tietz NW. Clinical Chemistry. Philadelphia, Pa: 2nd ed.,WB Saunders
    Co; 1994:1356, 1364, 1365.

(4) Simmons M, Sugahara K, Watanabe M. Development of an Improved
    Chloride Electrode for the Boehringer Mannheim/Hitachi 700 Series
    Analyzers. Poster session presented at the 1988 National Meeting
    of the American Association for Clinical Chemistry, New Orleans,
    LA, July 27, 1988 (reprints available from Roche Diagnostics
    Corporation).

(5) Young DS, et al. Clin Chem. 1975;21, No 5.

(6) Friedman RB, et al. Clin Chem. 1980;26, No 4.

Methodology:

Ion Selective Electrode

Sample Processing:

Centrifuge at a speed and time necessary to get barrier separation of plasma/serum and cells within 1 hour of collection. Send specimen in original tube. Do Not transfer to another tube.
Each sample must be labeled with at least TWO full patient identifiers (First/Last Name & DOB are sufficient for non-UIHC affiliated clients) to avoid sample rejection/delays.

Sample Storage:

Refrigerate.
All sample storage requirements are intended for delivery to UIHC within 24 hours of collection for testing. If samples won't arrive in this time period, please call the UIHC Core Lab for alternative storage/shipping instructions (319-356-3527).

Transport Instructions:

Place labeled specimen into zip-lock type biohazard bag; seal bag.
Place completed requisition into outside pocket of bag.
Transport in cooler with refrigerated coolant packs.